Abstract
Odorant receptors (ORs), recognized as the largest subfamily of G protein-coupled receptors (GPCR), are increasingly identified as membrane proteins crucial not only in nasal but also in various extra-nasal biological processes. However, researching the functions of these extra-nasal ORs is challenging due to the limited availability of ligands, posing a significant barrier to comprehensive studies. Large-scale screening with in vitro assays, such as the halide-sensitive yellow fluorescent protein (YFP) quenching assay, is crucial for deorphanizing ORs. This protocol employs the YFP quenching assay to identify OR-ligand interactions, thereby advancing OR deorphanization research.
| Original language | English |
|---|---|
| Title of host publication | Methods in Molecular Biology |
| Publisher | Humana Press Inc. |
| Pages | 169-177 |
| Number of pages | 9 |
| DOIs | |
| State | Published - 2025 |
Publication series
| Name | Methods in Molecular Biology |
|---|---|
| Volume | 2915 |
| ISSN (Print) | 1064-3745 |
| ISSN (Electronic) | 1940-6029 |
Bibliographical note
Publisher Copyright:© The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature 2025.
Keywords
- Extra-nasal OR functions
- Functional assays
- GPCR
- Ligand screening
- Membrane proteins
- OR deorphanization
- Odorant receptor
- Olfactory receptor
- Signal transduction
- YFP quenching assay