The HOOK region of β subunits controls gating of voltage-gated Ca2+ channels by electrostatically interacting with plasma membrane

Cheon Gyu Park, Byung Chang Suh

Research output: Contribution to journalArticlepeer-review

4 Scopus citations

Abstract

Recently, we showed that the HOOK region of the β2 subunit electrostatically interacts with the plasma membrane and regulates the current inactivation and phosphatidylinositol 4,5-bisphosphate (PIP2) sensitivity of voltage-gated Ca2+ (CaV) 2.2 channels. Here, we report that voltage-dependent gating and current density of the CaV2.2 channels are also regulated by the HOOK region of the β2 subunit. The HOOK region can be divided into 3 domains: S (polyserine), A (polyacidic), and B (polybasic). We found that the A domain shifted the voltage-dependent inactivation and activation of CaV2.2 channels to more hyperpolarized and depolarized voltages, respectively, whereas the B domain evoked these responses in the opposite directions. In addition, the A domain decreased the current density of the CaV2.2 channels, while the B domain increased it. Together, our data demonstrate that the flexible HOOK region of the β2 subunit plays an important role in determining the overall CaV channel gating properties.

Original languageEnglish
Pages (from-to)467-475
Number of pages9
JournalChannels (Austin, Tex.)
Volume11
Issue number5
DOIs
StatePublished - 3 Sep 2017

Keywords

  • HOOK region
  • Voltage-gated Ca2+ (CaV) channel
  • current density
  • electrostatic interaction
  • plasma membrane
  • voltage-dependent gating
  • β2c subunit

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