TY - JOUR
T1 - The HOOK region of β subunits controls gating of voltage-gated Ca2+ channels by electrostatically interacting with plasma membrane
AU - Park, Cheon Gyu
AU - Suh, Byung Chang
PY - 2017/9/3
Y1 - 2017/9/3
N2 - Recently, we showed that the HOOK region of the β2 subunit electrostatically interacts with the plasma membrane and regulates the current inactivation and phosphatidylinositol 4,5-bisphosphate (PIP2) sensitivity of voltage-gated Ca2+ (CaV) 2.2 channels. Here, we report that voltage-dependent gating and current density of the CaV2.2 channels are also regulated by the HOOK region of the β2 subunit. The HOOK region can be divided into 3 domains: S (polyserine), A (polyacidic), and B (polybasic). We found that the A domain shifted the voltage-dependent inactivation and activation of CaV2.2 channels to more hyperpolarized and depolarized voltages, respectively, whereas the B domain evoked these responses in the opposite directions. In addition, the A domain decreased the current density of the CaV2.2 channels, while the B domain increased it. Together, our data demonstrate that the flexible HOOK region of the β2 subunit plays an important role in determining the overall CaV channel gating properties.
AB - Recently, we showed that the HOOK region of the β2 subunit electrostatically interacts with the plasma membrane and regulates the current inactivation and phosphatidylinositol 4,5-bisphosphate (PIP2) sensitivity of voltage-gated Ca2+ (CaV) 2.2 channels. Here, we report that voltage-dependent gating and current density of the CaV2.2 channels are also regulated by the HOOK region of the β2 subunit. The HOOK region can be divided into 3 domains: S (polyserine), A (polyacidic), and B (polybasic). We found that the A domain shifted the voltage-dependent inactivation and activation of CaV2.2 channels to more hyperpolarized and depolarized voltages, respectively, whereas the B domain evoked these responses in the opposite directions. In addition, the A domain decreased the current density of the CaV2.2 channels, while the B domain increased it. Together, our data demonstrate that the flexible HOOK region of the β2 subunit plays an important role in determining the overall CaV channel gating properties.
KW - HOOK region
KW - Voltage-gated Ca2+ (CaV) channel
KW - current density
KW - electrostatic interaction
KW - plasma membrane
KW - voltage-dependent gating
KW - β2c subunit
UR - http://www.scopus.com/inward/record.url?scp=85021216967&partnerID=8YFLogxK
U2 - 10.1080/19336950.2017.1335841
DO - 10.1080/19336950.2017.1335841
M3 - Article
C2 - 28569643
AN - SCOPUS:85021216967
SN - 1933-6950
VL - 11
SP - 467
EP - 475
JO - Channels (Austin, Tex.)
JF - Channels (Austin, Tex.)
IS - 5
ER -