Stable small quantum dots for synaptic receptor tracking on live neurons

En Cai; Pinghua Ge; Sang Hak Lee; Okunola Jeyifous; Yong Wang; Yanxin Liu; Katie M. Wilson; Sung Jun Lim; Michelle A. Baird; John E. Stone; Kwan Young Lee; Michael W. Davidson; Hee Jung Chung; Klaus Schulten; Andrew M. Smith; William N. Green; Paul R. Selvin

doi:10.1002/anie.201405735

Stable small quantum dots for synaptic receptor tracking on live neurons

En Cai, Pinghua Ge, Sang Hak Lee, Okunola Jeyifous, Yong Wang, Yanxin Liu, Katie M. Wilson, Sung Jun Lim, Michelle A. Baird, John E. Stone, Kwan Young Lee, Michael W. Davidson, Hee Jung Chung, Klaus Schulten, Andrew M. Smith, William N. Green, Paul R. Selvin

Division of Nanotechnology

Research output: Contribution to journal › Article › peer-review

63 Scopus citations

Abstract

We developed a coating method to produce functionalized small quantum dots (sQDs), about 9 nmin diameter, that were stable for over a month. We made sQDs in four emission wavelengths, from 527 to 655 nm and with different functional groups. AMPA receptors on live neurons were labeled with sQDs and postsynaptic density proteins were visualized with super-resolution microscopy. Their diffusion behavior indicates that sQDs access the synaptic clefts significantly more often than commercial QDs.

Original language	English
Pages (from-to)	12484-12488
Number of pages	5
Journal	Angewandte Chemie - International Edition
Volume	53
Issue number	46
DOIs	https://doi.org/10.1002/anie.201405735
State	Published - 10 Nov 2014

Bibliographical note

Publisher Copyright:
© 2014 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim.

Keywords

AMPA receptors
Fluorescent probes
Quantum dots
Single-molecule studies

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10.1002/anie.201405735

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Cai, E., Ge, P., Lee, S. H., Jeyifous, O., Wang, Y., Liu, Y., Wilson, K. M., Lim, S. J., Baird, M. A., Stone, J. E., Lee, K. Y., Davidson, M. W., Chung, H. J., Schulten, K., Smith, A. M., Green, W. N., & Selvin, P. R. (2014). Stable small quantum dots for synaptic receptor tracking on live neurons. Angewandte Chemie - International Edition, 53(46), 12484-12488. https://doi.org/10.1002/anie.201405735

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title = "Stable small quantum dots for synaptic receptor tracking on live neurons",

abstract = "We developed a coating method to produce functionalized small quantum dots (sQDs), about 9 nmin diameter, that were stable for over a month. We made sQDs in four emission wavelengths, from 527 to 655 nm and with different functional groups. AMPA receptors on live neurons were labeled with sQDs and postsynaptic density proteins were visualized with super-resolution microscopy. Their diffusion behavior indicates that sQDs access the synaptic clefts significantly more often than commercial QDs.",

keywords = "AMPA receptors, Fluorescent probes, Quantum dots, Single-molecule studies",

author = "En Cai and Pinghua Ge and Lee, {Sang Hak} and Okunola Jeyifous and Yong Wang and Yanxin Liu and Wilson, {Katie M.} and Lim, {Sung Jun} and Baird, {Michelle A.} and Stone, {John E.} and Lee, {Kwan Young} and Davidson, {Michael W.} and Chung, {Hee Jung} and Klaus Schulten and Smith, {Andrew M.} and Green, {William N.} and Selvin, {Paul R.}",

note = "Publisher Copyright: {\textcopyright} 2014 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim.",

year = "2014",

month = nov,

day = "10",

doi = "10.1002/anie.201405735",

language = "English",

volume = "53",

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journal = "Angewandte Chemie - International Edition",

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Cai, E, Ge, P, Lee, SH, Jeyifous, O, Wang, Y, Liu, Y, Wilson, KM, Lim, SJ, Baird, MA, Stone, JE, Lee, KY, Davidson, MW, Chung, HJ, Schulten, K, Smith, AM, Green, WN & Selvin, PR 2014, 'Stable small quantum dots for synaptic receptor tracking on live neurons', Angewandte Chemie - International Edition, vol. 53, no. 46, pp. 12484-12488. https://doi.org/10.1002/anie.201405735

TY - JOUR

T1 - Stable small quantum dots for synaptic receptor tracking on live neurons

AU - Cai, En

AU - Ge, Pinghua

AU - Lee, Sang Hak

AU - Jeyifous, Okunola

AU - Wang, Yong

AU - Liu, Yanxin

AU - Wilson, Katie M.

AU - Lim, Sung Jun

AU - Baird, Michelle A.

AU - Stone, John E.

AU - Lee, Kwan Young

AU - Davidson, Michael W.

AU - Chung, Hee Jung

AU - Schulten, Klaus

AU - Smith, Andrew M.

AU - Green, William N.

AU - Selvin, Paul R.

PY - 2014/11/10

Y1 - 2014/11/10

N2 - We developed a coating method to produce functionalized small quantum dots (sQDs), about 9 nmin diameter, that were stable for over a month. We made sQDs in four emission wavelengths, from 527 to 655 nm and with different functional groups. AMPA receptors on live neurons were labeled with sQDs and postsynaptic density proteins were visualized with super-resolution microscopy. Their diffusion behavior indicates that sQDs access the synaptic clefts significantly more often than commercial QDs.

AB - We developed a coating method to produce functionalized small quantum dots (sQDs), about 9 nmin diameter, that were stable for over a month. We made sQDs in four emission wavelengths, from 527 to 655 nm and with different functional groups. AMPA receptors on live neurons were labeled with sQDs and postsynaptic density proteins were visualized with super-resolution microscopy. Their diffusion behavior indicates that sQDs access the synaptic clefts significantly more often than commercial QDs.

KW - AMPA receptors

KW - Fluorescent probes

KW - Quantum dots

KW - Single-molecule studies

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U2 - 10.1002/anie.201405735

DO - 10.1002/anie.201405735

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SN - 1433-7851

VL - 53

SP - 12484

EP - 12488

JO - Angewandte Chemie - International Edition

JF - Angewandte Chemie - International Edition

IS - 46

ER -

Stable small quantum dots for synaptic receptor tracking on live neurons

Abstract

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Keywords

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