Senescence marker protein 30 (Smp30): A novel pan-species diagnostic marker for the histopathological diagnosis of breast cancer in humans and animals

Su Min Baek, Seoung Woo Lee, Tae Un Kim, Seong Kyoon Choi, Sungho Yun, Won Jae Lee, Se Hyeon Han, Il Hwa Hong, Sang Joon Park, Tae Hwan Kim, Kyu Shik Jeong, Jin Kyu Park

Research output: Contribution to journalArticlepeer-review

5 Scopus citations

Abstract

Senescence marker protein 30 (SMP30) is a cell survival factor playing an important role in vitamin C synthesis and antiapoptosis. Moreover, its cytoprotective role suggests a possibility to be related to cancer cell survival. Mammary carcinoma is a common cancer in both humans and animals. Because of its histopathological diversity, especially in the early stage, histopathological diagnosis may be complicated; therefore, a diagnostic marker is helpful for confirmation. The present study analyzed the expression pattern of SMP30 in mammary carcinoma in humans, dogs, and cats. Immunohistochemistry, immunofluorescence, and western blot analysis were used to investigate SMP30 expression patterns. The expression was specifically observed in neoplastic glandular epithelial cells. The expression increased with the malignancy of glandular epithelial cells with a highly proliferative status. However, SMP30 expression was low in normal mammary gland tissues or well-differentiated adenoma tissues. The patterns were consistently reproduced in canine primary mammary carcinoma cells and MCF-7 and MDA-MB-231 human carcinoma cell lines. This study provides useful information to understand SMP30 expression in various stages of mammary carcinoma and to suggest its utility as a pan-species diagnostic marker, thereby helping to establish strategies for diagnosing mammary carcinoma in several species.

Original languageEnglish
Article number2340
Pages (from-to)1-18
Number of pages18
JournalInternational Journal of Molecular Sciences
Volume22
Issue number5
DOIs
StatePublished - 1 Mar 2021

Bibliographical note

Publisher Copyright:
© 2021 by the authors. Licensee MDPI, Basel, Switzerland.

Keywords

  • Breast cancer
  • Cat
  • Diagnostic marker
  • Dog
  • Human
  • Mammary carcinoma
  • Neoplastic glandular epithelial cell
  • SMP30

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