@inproceedings{fdf509f97fe04e89a13461e6080426d7,
title = "Real-time detection of neurite outgrowth using microfluidic device",
abstract = "We developed a simple method for real-time detection of the neurite outgrowth using microfluidic device. Our microfluidic device contains three compartmentalized channels which are for cell seeding, hydrogel and growth factors. Collagen gel is filled in the middle channel and pheochromocytoma (PC12) cells are seeded in the left channel. To induce differentiation of PC12 cells, 50 ng/ml to1000 ng/ml of nerve growth factor (NGF) is introduced into the right channel. After three days of NGF treatment, PC12 cells begin to extend neurites and formed neurite network from sixth day. Quantification of neurite outgrowth is analyzed by measuring the total area of neurites. On sixth day, the area is doubled compared to the area on third day and increases by 20 times on ninth day.",
keywords = "Microfluidic device, Nerve growth factor, Neurite outgrowth",
author = "Samhwan Kim and Jongmoon Jang and Hongsoo Choi and Cheil Moon",
year = "2013",
doi = "10.1117/12.2018667",
language = "English",
isbn = "9780819493040",
series = "Proceedings of SPIE - The International Society for Optical Engineering",
booktitle = "Nano-Bio Sensing, Imaging, and Spectroscopy",
note = "SPIE Nano-Bio Sensing, Imaging, and Spectroscopy ; Conference date: 20-02-2013 Through 23-02-2013",
}