Ratiometric spectral imaging for fast tumor detection and chemotherapy monitoring in vivo

Jae Youn Hwang, Zeev Gross, Harry B. Gray, Lali K. Medina-Kauwe, Daniel L. Farkas

Research output: Contribution to journalArticlepeer-review

19 Scopus citations

Abstract

We report a novel in vivo spectral imaging approach to cancer detection and chemotherapy assessment. We describe and characterize a ratiometric spectral imaging and analysis method and evaluate its performance for tumor detection and delineation by quantitatively monitoring the specific accumulation of targeted gallium corrole (HerGa) into HER2-positive (HER2+) breast tumors. HerGa temporal accumulation in nude mice bearing HER2+ breast tumors was monitored comparatively by a. this new ratiometric imaging and analysis method; b. established (reflectance and fluorescence) spectral imaging; c. more commonly used fluorescence intensity imaging. We also tested the feasibility of HerGa imaging in vivo using the ratiometric spectral imaging method for tumor detection and delineation. Our results show that the new method not only provides better quantitative information than typical spectral imaging, but also better specificity than standard fluorescence intensity imaging, thus allowing enhanced in vivo outlining of tumors and dynamic, quantitative monitoring of targeted chemotherapy agent accumulation into them.

Original languageEnglish
Article number066007
JournalJournal of Biomedical Optics
Volume16
Issue number6
DOIs
StatePublished - Jun 2011

Bibliographical note

Funding Information:
This research was supported by the US Navy Bureau of Medicine and Surgery (D.L.F.), the NIH DK019038 (H.B.G.), the NIH R01 CA140995 (L.K.M.K.), the NIH R01 CA129822 (L.K.M.K.), and the BSF (Z.G. and H.B.G.). We sincerely thank Dr. V. Krishnan Ramanujan for providing valuable comments and Dr. Jihoon Jeong for developing and providing an ImageJ plug-in program for spectral imaging.

Keywords

  • Chemotherapy
  • Ratiometric
  • Spectral imaging
  • Tumor detection
  • Tumor-targeted gallium corrole

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