Quantitative ToF-SIMS study of surface-immobilized streptavidin

Young Pil Kim; Mi Young Hong; Hyun Kyong Shon; Dae Won Moon; Hak Sung Kim; Tae Geol Lee

doi:10.1016/j.apsusc.2006.02.203

Quantitative ToF-SIMS study of surface-immobilized streptavidin

Young Pil Kim, Mi Young Hong, Hyun Kyong Shon, Dae Won Moon, Hak Sung Kim, Tae Geol Lee

Department of New Biology

Research output: Contribution to journal › Article › peer-review

26 Scopus citations

Abstract

ToF-SIMS analysis with principal component analysis (PCA) has been used for quantitatively studying the interaction between streptavidin and biotin on a dendrimer surface. A poly(amidoamine) dendrimer surface was used as a model amine surface for biotinylation. The surface streptavidin density was systematically varied and independently quantified using the surface plasmon resonance (SPR) technique. A good linear correlation of streptavidin density was observed between the ToF-SIMS and SPR results.

Original language	English
Pages (from-to)	6801-6804
Number of pages	4
Journal	Applied Surface Science
Volume	252
Issue number	19
DOIs	https://doi.org/10.1016/j.apsusc.2006.02.203
State	Published - 30 Jul 2006

Bibliographical note

Funding Information:
This work was supported by the R&D Program of Fusion Strategies for Advanced Technologies of MOCIE.

Keywords

Dendrimer
PCA
Quantification
SPR
Streptavidin
ToF-SIMS

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10.1016/j.apsusc.2006.02.203

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title = "Quantitative ToF-SIMS study of surface-immobilized streptavidin",

abstract = "ToF-SIMS analysis with principal component analysis (PCA) has been used for quantitatively studying the interaction between streptavidin and biotin on a dendrimer surface. A poly(amidoamine) dendrimer surface was used as a model amine surface for biotinylation. The surface streptavidin density was systematically varied and independently quantified using the surface plasmon resonance (SPR) technique. A good linear correlation of streptavidin density was observed between the ToF-SIMS and SPR results.",

keywords = "Dendrimer, PCA, Quantification, SPR, Streptavidin, ToF-SIMS",

author = "Kim, {Young Pil} and Hong, {Mi Young} and Shon, {Hyun Kyong} and Moon, {Dae Won} and Kim, {Hak Sung} and Lee, {Tae Geol}",

note = "Funding Information: This work was supported by the R&D Program of Fusion Strategies for Advanced Technologies of MOCIE.",

year = "2006",

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doi = "10.1016/j.apsusc.2006.02.203",

language = "English",

volume = "252",

pages = "6801--6804",

journal = "Applied Surface Science",

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T1 - Quantitative ToF-SIMS study of surface-immobilized streptavidin

AU - Kim, Young Pil

AU - Hong, Mi Young

AU - Shon, Hyun Kyong

AU - Moon, Dae Won

AU - Kim, Hak Sung

AU - Lee, Tae Geol

N1 - Funding Information: This work was supported by the R&D Program of Fusion Strategies for Advanced Technologies of MOCIE.

PY - 2006/7/30

Y1 - 2006/7/30

N2 - ToF-SIMS analysis with principal component analysis (PCA) has been used for quantitatively studying the interaction between streptavidin and biotin on a dendrimer surface. A poly(amidoamine) dendrimer surface was used as a model amine surface for biotinylation. The surface streptavidin density was systematically varied and independently quantified using the surface plasmon resonance (SPR) technique. A good linear correlation of streptavidin density was observed between the ToF-SIMS and SPR results.

AB - ToF-SIMS analysis with principal component analysis (PCA) has been used for quantitatively studying the interaction between streptavidin and biotin on a dendrimer surface. A poly(amidoamine) dendrimer surface was used as a model amine surface for biotinylation. The surface streptavidin density was systematically varied and independently quantified using the surface plasmon resonance (SPR) technique. A good linear correlation of streptavidin density was observed between the ToF-SIMS and SPR results.

KW - Dendrimer

KW - PCA

KW - Quantification

KW - SPR

KW - Streptavidin

KW - ToF-SIMS

UR - http://www.scopus.com/inward/record.url?scp=33747192313&partnerID=8YFLogxK

U2 - 10.1016/j.apsusc.2006.02.203

DO - 10.1016/j.apsusc.2006.02.203

M3 - Article

AN - SCOPUS:33747192313

SN - 0169-4332

VL - 252

SP - 6801

EP - 6804

JO - Applied Surface Science

JF - Applied Surface Science

IS - 19

ER -

Quantitative ToF-SIMS study of surface-immobilized streptavidin

Abstract

Bibliographical note

Keywords

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