Abstract
Quantitative phase information obtained by digital holographic microscopy (DHM) can provide new insight into the functions and morphology of single red blood cells (RBCs). Since the functionality of a RBC is related to its three-dimensional (3-D) shape, quantitative 3-D geometric changes induced by storage time can help hematologists realize its optimal functionality period. We quantitatively investigate RBC 3-D geometric changes in the storage lesion using DHM. Our experimental results show that the substantial geometric transformation of the biconcave-shaped RBCs to the spherocyte occurs due to RBC storage lesion. This transformation leads to progressive loss of cell surface area, surface-to-volume ratio, and functionality of RBCs. Furthermore, our quantitative analysis shows that there are significant correlations between chemical and morphological properties of RBCs.
| Original language | English |
|---|---|
| Article number | 111218 |
| Journal | Journal of Biomedical Optics |
| Volume | 20 |
| Issue number | 11 |
| DOIs | |
| State | Published - 1 Nov 2015 |
Bibliographical note
Publisher Copyright:© 2015 Society of Photo-Optical Instrumentation Engineers (SPIE).
Keywords
- cell morphology analysis
- digital holographic microscopy
- quantitative phase imaging
- red blood cell analysis
- three-dimensional image processing