Polyaniline - Carboxymethyl cellulose nanocomposite for cholesterol detection

Abdul Barik; Pratima R. Solanki; Ajeet Kaushik; Azahar Ali; M. K. Pandey; C. G. Kim; B. D. Malhotra

doi:10.1166/jnn.2010.2511

Polyaniline - Carboxymethyl cellulose nanocomposite for cholesterol detection

Abdul Barik, Pratima R. Solanki, Ajeet Kaushik, Azahar Ali, M. K. Pandey, C. G. Kim, B. D. Malhotra

Department of Physics and Chemistry

Research output: Contribution to journal › Article › peer-review

33 Scopus citations

Abstract

Cholesterol oxidase (ChOx) has been covalently immobilized onto polyaniline-carboxymethyl cel-lulose (PANI-CMC) nanocomposite film deposited onto indium-tin-oxide (ITO) coated glass plate using glutaraldehyde as a cross-linker. Fourier transform infrared (FTIR) spectroscopic and electro-chemical studies have been used to characterize the PANI-CMC/ITO nanocomposite electrode and ChOx/PANI-CMC/ITO bioelectrode. Scanning electron microscopy (SEM) studies reveal the formation of PANI-CMC nanocomposite fibers of size ∼150 nm in diameter. The ChOx/PANI-CMC/ITO bioelectrode exhibits linearity as 0.5-22 mM, detection limit as 1.31 mM, sensitivity as 0.14 mA/mM cm ², response time as 10 s and shelf-life of about 10 weeks when bioelectrode is stored at 4 °C. The low value of Michaelis-Menten constant (K _m) obtained as 2.71 mM reveals high affinity of immobilized ChOx for PANI-CMC/ITO nanocomposite electrode.

Original language	English
Pages (from-to)	6479-6488
Number of pages	10
Journal	Journal of Nanoscience and Nanotechnology
Volume	10
Issue number	10
DOIs	https://doi.org/10.1166/jnn.2010.2511
State	Published - Oct 2010

Keywords

Biosensor
Carboxymethyl cellulose
Cholesterol
Cholesterol oxidase
Electrochemical polymerization
Polyaniline

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10.1166/jnn.2010.2511

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@article{66305048cb65441b8686d068c8e675a6,

title = "Polyaniline - Carboxymethyl cellulose nanocomposite for cholesterol detection",

abstract = "Cholesterol oxidase (ChOx) has been covalently immobilized onto polyaniline-carboxymethyl cel-lulose (PANI-CMC) nanocomposite film deposited onto indium-tin-oxide (ITO) coated glass plate using glutaraldehyde as a cross-linker. Fourier transform infrared (FTIR) spectroscopic and electro-chemical studies have been used to characterize the PANI-CMC/ITO nanocomposite electrode and ChOx/PANI-CMC/ITO bioelectrode. Scanning electron microscopy (SEM) studies reveal the formation of PANI-CMC nanocomposite fibers of size ∼150 nm in diameter. The ChOx/PANI-CMC/ITO bioelectrode exhibits linearity as 0.5-22 mM, detection limit as 1.31 mM, sensitivity as 0.14 mA/mM cm 2, response time as 10 s and shelf-life of about 10 weeks when bioelectrode is stored at 4 °C. The low value of Michaelis-Menten constant (K m) obtained as 2.71 mM reveals high affinity of immobilized ChOx for PANI-CMC/ITO nanocomposite electrode.",

keywords = "Biosensor, Carboxymethyl cellulose, Cholesterol, Cholesterol oxidase, Electrochemical polymerization, Polyaniline",

author = "Abdul Barik and Solanki, {Pratima R.} and Ajeet Kaushik and Azahar Ali and Pandey, {M. K.} and Kim, {C. G.} and Malhotra, {B. D.}",

year = "2010",

month = oct,

doi = "10.1166/jnn.2010.2511",

language = "English",

volume = "10",

pages = "6479--6488",

journal = "Journal of Nanoscience and Nanotechnology",

issn = "1533-4880",

publisher = "American Scientific Publishers",

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T1 - Polyaniline - Carboxymethyl cellulose nanocomposite for cholesterol detection

AU - Barik, Abdul

AU - Solanki, Pratima R.

AU - Kaushik, Ajeet

AU - Ali, Azahar

AU - Pandey, M. K.

AU - Kim, C. G.

AU - Malhotra, B. D.

PY - 2010/10

Y1 - 2010/10

N2 - Cholesterol oxidase (ChOx) has been covalently immobilized onto polyaniline-carboxymethyl cel-lulose (PANI-CMC) nanocomposite film deposited onto indium-tin-oxide (ITO) coated glass plate using glutaraldehyde as a cross-linker. Fourier transform infrared (FTIR) spectroscopic and electro-chemical studies have been used to characterize the PANI-CMC/ITO nanocomposite electrode and ChOx/PANI-CMC/ITO bioelectrode. Scanning electron microscopy (SEM) studies reveal the formation of PANI-CMC nanocomposite fibers of size ∼150 nm in diameter. The ChOx/PANI-CMC/ITO bioelectrode exhibits linearity as 0.5-22 mM, detection limit as 1.31 mM, sensitivity as 0.14 mA/mM cm 2, response time as 10 s and shelf-life of about 10 weeks when bioelectrode is stored at 4 °C. The low value of Michaelis-Menten constant (K m) obtained as 2.71 mM reveals high affinity of immobilized ChOx for PANI-CMC/ITO nanocomposite electrode.

AB - Cholesterol oxidase (ChOx) has been covalently immobilized onto polyaniline-carboxymethyl cel-lulose (PANI-CMC) nanocomposite film deposited onto indium-tin-oxide (ITO) coated glass plate using glutaraldehyde as a cross-linker. Fourier transform infrared (FTIR) spectroscopic and electro-chemical studies have been used to characterize the PANI-CMC/ITO nanocomposite electrode and ChOx/PANI-CMC/ITO bioelectrode. Scanning electron microscopy (SEM) studies reveal the formation of PANI-CMC nanocomposite fibers of size ∼150 nm in diameter. The ChOx/PANI-CMC/ITO bioelectrode exhibits linearity as 0.5-22 mM, detection limit as 1.31 mM, sensitivity as 0.14 mA/mM cm 2, response time as 10 s and shelf-life of about 10 weeks when bioelectrode is stored at 4 °C. The low value of Michaelis-Menten constant (K m) obtained as 2.71 mM reveals high affinity of immobilized ChOx for PANI-CMC/ITO nanocomposite electrode.

KW - Biosensor

KW - Carboxymethyl cellulose

KW - Cholesterol

KW - Cholesterol oxidase

KW - Electrochemical polymerization

KW - Polyaniline

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Polyaniline - Carboxymethyl cellulose nanocomposite for cholesterol detection

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