TY - GEN
T1 - Planar hall effect in spin valve structure for DNA detection immobilized with single magnetic bead
AU - Bajaj, Bharat
AU - Thanh, N. T.
AU - Kim, C. G.
PY - 2007
Y1 - 2007
N2 - There is a considerable effort directed towards understanding and negating the proliferation of infectious diseases by improving the sensitivity of biosensors. We describe the investigation of nucleic acid interactions based on immobilization of thiolated probe DNA followed by the coupling of biotinylated DNA and streptavidin coated Dyna beads on the gold film deposited on the substrate for the further application with the Planar Hall effect in spin valve structure of Ta/NiFe/Cu/MFe/IrMn/Ta which has been applied to single magnetic bead detection sensor with the size of 3×3μm2. The spin valve structure which incorporates free and pinned layers was used as magnetic field sensor based on the resistance changes when the magnetization orientation in free magnetic layer rotates to the change of external applied magnetic field. It is demonstrated to induce a high sensitivity due to small interlayer coupling between free and pinned layers, and improved signal-to-noise due to very thin active sensing layer; compared to the other structures.. In the results, the sensor performance with single Dynabeads® M-280 Streptavidin conjugated with target DNA and probe DNA was reported as a significantly sensitive system with high signals of around 1.4 μV. These, therefore, can be used well for biomolecule recognition, biotechnology and biosensor application.
AB - There is a considerable effort directed towards understanding and negating the proliferation of infectious diseases by improving the sensitivity of biosensors. We describe the investigation of nucleic acid interactions based on immobilization of thiolated probe DNA followed by the coupling of biotinylated DNA and streptavidin coated Dyna beads on the gold film deposited on the substrate for the further application with the Planar Hall effect in spin valve structure of Ta/NiFe/Cu/MFe/IrMn/Ta which has been applied to single magnetic bead detection sensor with the size of 3×3μm2. The spin valve structure which incorporates free and pinned layers was used as magnetic field sensor based on the resistance changes when the magnetization orientation in free magnetic layer rotates to the change of external applied magnetic field. It is demonstrated to induce a high sensitivity due to small interlayer coupling between free and pinned layers, and improved signal-to-noise due to very thin active sensing layer; compared to the other structures.. In the results, the sensor performance with single Dynabeads® M-280 Streptavidin conjugated with target DNA and probe DNA was reported as a significantly sensitive system with high signals of around 1.4 μV. These, therefore, can be used well for biomolecule recognition, biotechnology and biosensor application.
KW - Bio sensor
KW - Dynabead
KW - Magnetic bead detection
UR - http://www.scopus.com/inward/record.url?scp=52949090064&partnerID=8YFLogxK
U2 - 10.1109/NANO.2007.4601359
DO - 10.1109/NANO.2007.4601359
M3 - Conference contribution
AN - SCOPUS:52949090064
SN - 1424406080
SN - 9781424406081
T3 - 2007 7th IEEE International Conference on Nanotechnology - IEEE-NANO 2007, Proceedings
SP - 1033
EP - 1036
BT - 2007 7th IEEE International Conference on Nanotechnology - IEEE-NANO 2007, Proceedings
T2 - 2007 7th IEEE International Conference on Nanotechnology - IEEE-NANO 2007
Y2 - 2 August 2007 through 5 August 2007
ER -