Phosphorylation of the Arabidopsis AtrbohF NADPH oxidase by OST1 protein kinase

Caroline Sirichandra; Dan Gu; Heng Cheng Hu; Marlène Davanture; Sangmee Lee; Michaël Djaoui; Benoît Valot; Michel Zivy; Jeffrey Leung; Sylvain Merlot; June M. Kwak

doi:10.1016/j.febslet.2009.08.033

Phosphorylation of the Arabidopsis AtrbohF NADPH oxidase by OST1 protein kinase

Caroline Sirichandra
, Dan Gu
, Heng Cheng Hu
, Marlène Davanture
, Sangmee Lee
, Michaël Djaoui
, Benoît Valot
, Michel Zivy
, Jeffrey Leung
, Sylvain Merlot
, June M. Kwak

Department of New Biology

Research output: Contribution to journal › Article › peer-review

398 Scopus citations

Abstract

The plant hormone abscisic acid (ABA) triggers production of reactive oxygen species (ROS) in guard cells via the AtrbohD and AtrbohF NADPH oxidases, leading to stomatal closure. The ABA-activated SnRK2 protein kinase open stomata 1 (OST1) (SRK2E/SnRK2.6) acts upstream of ROS in guard cell ABA signaling. Here, we report that OST1 phosphorylates Ser13 and Ser174 on AtrbohF. In addition, substitution of Ser174 to Ala results in a ∼40% reduction in the phosphorylation of AtrbohF by OST1. We also show that OST1 physically interacts with AtrbohF. These results provide biochemical evidence suggesting that OST1 regulates AtrbohF activity. Structured summary: MINT-7260179, MINT-7260147, MINT-7260165: OST1 (uniprotkb:Q940H6) phosphorylates (MI:0217) ATRBOHF (uniprotkb:O48538) by protein kinase assay (MI:0424). MINT-7260208: OST1 (uniprotkb:Q940H6) and ATRBOHF (uniprotkb:O48538) physically interact (MI:0915) by bimolecular fluorescence complementation (MI:0809).

Original language	English
Pages (from-to)	2982-2986
Number of pages	5
Journal	FEBS Letters
Volume	583
Issue number	18
DOIs	https://doi.org/10.1016/j.febslet.2009.08.033
State	Published - 17 Sep 2009

Bibliographical note

Funding Information:
We thank A.C. Mustilli, S. Thomine, and C. Belin for kindly providing the GFP-OST1 construct. We also thank Drs. C. Chang, H. Sze, and Z. Liu for critical reading of the manuscript and J. Giraudat for technical help. This work was supported by NRI Grants ( 2004-35100-14909 , 2007-35100-18377 ) from the USDA CSREES and in part by a Grant ( MCB-0618402 ) from the NSF to J.M.K., and in part by Centre National de la Recherche Scientifique to S.M. and J.L. C.S. was supported by a doctoral fellowship from the French Ministère de l’Enseignement Supérieur et de la Recherche.

Keywords

Abscisic acid
Guard cell
Protein kinase
Reactive oxygen species

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10.1016/j.febslet.2009.08.033

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@article{26195c701f264f449e8374a87f44bffe,

title = "Phosphorylation of the Arabidopsis AtrbohF NADPH oxidase by OST1 protein kinase",

abstract = "The plant hormone abscisic acid (ABA) triggers production of reactive oxygen species (ROS) in guard cells via the AtrbohD and AtrbohF NADPH oxidases, leading to stomatal closure. The ABA-activated SnRK2 protein kinase open stomata 1 (OST1) (SRK2E/SnRK2.6) acts upstream of ROS in guard cell ABA signaling. Here, we report that OST1 phosphorylates Ser13 and Ser174 on AtrbohF. In addition, substitution of Ser174 to Ala results in a ∼40\% reduction in the phosphorylation of AtrbohF by OST1. We also show that OST1 physically interacts with AtrbohF. These results provide biochemical evidence suggesting that OST1 regulates AtrbohF activity. Structured summary: MINT-7260179, MINT-7260147, MINT-7260165: OST1 (uniprotkb:Q940H6) phosphorylates (MI:0217) ATRBOHF (uniprotkb:O48538) by protein kinase assay (MI:0424). MINT-7260208: OST1 (uniprotkb:Q940H6) and ATRBOHF (uniprotkb:O48538) physically interact (MI:0915) by bimolecular fluorescence complementation (MI:0809).",

keywords = "Abscisic acid, Guard cell, Protein kinase, Reactive oxygen species",

author = "Caroline Sirichandra and Dan Gu and Hu, \{Heng Cheng\} and Marl{\`e}ne Davanture and Sangmee Lee and Micha{\"e}l Djaoui and Beno{\^i}t Valot and Michel Zivy and Jeffrey Leung and Sylvain Merlot and Kwak, \{June M.\}",

note = "Funding Information: We thank A.C. Mustilli, S. Thomine, and C. Belin for kindly providing the GFP-OST1 construct. We also thank Drs. C. Chang, H. Sze, and Z. Liu for critical reading of the manuscript and J. Giraudat for technical help. This work was supported by NRI Grants ( 2004-35100-14909 , 2007-35100-18377 ) from the USDA CSREES and in part by a Grant ( MCB-0618402 ) from the NSF to J.M.K., and in part by Centre National de la Recherche Scientifique to S.M. and J.L. C.S. was supported by a doctoral fellowship from the French Minist{\`e}re de l{\textquoteright}Enseignement Sup{\'e}rieur et de la Recherche. ",

year = "2009",

month = sep,

day = "17",

doi = "10.1016/j.febslet.2009.08.033",

language = "English",

volume = "583",

pages = "2982--2986",

journal = "FEBS Letters",

issn = "0014-5793",

publisher = "John Wiley and Sons Inc.",

number = "18",

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TY - JOUR

T1 - Phosphorylation of the Arabidopsis AtrbohF NADPH oxidase by OST1 protein kinase

AU - Sirichandra, Caroline

AU - Gu, Dan

AU - Hu, Heng Cheng

AU - Davanture, Marlène

AU - Lee, Sangmee

AU - Djaoui, Michaël

AU - Valot, Benoît

AU - Zivy, Michel

AU - Leung, Jeffrey

AU - Merlot, Sylvain

AU - Kwak, June M.

N1 - Funding Information: We thank A.C. Mustilli, S. Thomine, and C. Belin for kindly providing the GFP-OST1 construct. We also thank Drs. C. Chang, H. Sze, and Z. Liu for critical reading of the manuscript and J. Giraudat for technical help. This work was supported by NRI Grants ( 2004-35100-14909 , 2007-35100-18377 ) from the USDA CSREES and in part by a Grant ( MCB-0618402 ) from the NSF to J.M.K., and in part by Centre National de la Recherche Scientifique to S.M. and J.L. C.S. was supported by a doctoral fellowship from the French Ministère de l’Enseignement Supérieur et de la Recherche.

PY - 2009/9/17

Y1 - 2009/9/17

N2 - The plant hormone abscisic acid (ABA) triggers production of reactive oxygen species (ROS) in guard cells via the AtrbohD and AtrbohF NADPH oxidases, leading to stomatal closure. The ABA-activated SnRK2 protein kinase open stomata 1 (OST1) (SRK2E/SnRK2.6) acts upstream of ROS in guard cell ABA signaling. Here, we report that OST1 phosphorylates Ser13 and Ser174 on AtrbohF. In addition, substitution of Ser174 to Ala results in a ∼40% reduction in the phosphorylation of AtrbohF by OST1. We also show that OST1 physically interacts with AtrbohF. These results provide biochemical evidence suggesting that OST1 regulates AtrbohF activity. Structured summary: MINT-7260179, MINT-7260147, MINT-7260165: OST1 (uniprotkb:Q940H6) phosphorylates (MI:0217) ATRBOHF (uniprotkb:O48538) by protein kinase assay (MI:0424). MINT-7260208: OST1 (uniprotkb:Q940H6) and ATRBOHF (uniprotkb:O48538) physically interact (MI:0915) by bimolecular fluorescence complementation (MI:0809).

AB - The plant hormone abscisic acid (ABA) triggers production of reactive oxygen species (ROS) in guard cells via the AtrbohD and AtrbohF NADPH oxidases, leading to stomatal closure. The ABA-activated SnRK2 protein kinase open stomata 1 (OST1) (SRK2E/SnRK2.6) acts upstream of ROS in guard cell ABA signaling. Here, we report that OST1 phosphorylates Ser13 and Ser174 on AtrbohF. In addition, substitution of Ser174 to Ala results in a ∼40% reduction in the phosphorylation of AtrbohF by OST1. We also show that OST1 physically interacts with AtrbohF. These results provide biochemical evidence suggesting that OST1 regulates AtrbohF activity. Structured summary: MINT-7260179, MINT-7260147, MINT-7260165: OST1 (uniprotkb:Q940H6) phosphorylates (MI:0217) ATRBOHF (uniprotkb:O48538) by protein kinase assay (MI:0424). MINT-7260208: OST1 (uniprotkb:Q940H6) and ATRBOHF (uniprotkb:O48538) physically interact (MI:0915) by bimolecular fluorescence complementation (MI:0809).

KW - Abscisic acid

KW - Guard cell

KW - Protein kinase

KW - Reactive oxygen species

UR - https://www.scopus.com/pages/publications/70249117473

U2 - 10.1016/j.febslet.2009.08.033

DO - 10.1016/j.febslet.2009.08.033

M3 - Article

C2 - 19716822

AN - SCOPUS:70249117473

SN - 0014-5793

VL - 583

SP - 2982

EP - 2986

JO - FEBS Letters

JF - FEBS Letters

IS - 18

ER -

Phosphorylation of the Arabidopsis AtrbohF NADPH oxidase by OST1 protein kinase

Abstract

Bibliographical note

Keywords

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