Phosphoinositide 5- and 3-phosphatase activities of a voltage-sensing phosphatase in living cells show identical voltage dependence

Dongil Keuma, Martin Kruseb, Dong Il Kima, Bertil Hilleb, Byung Chang Suhb

Research output: Contribution to journalArticlepeer-review

31 Scopus citations

Abstract

Voltage-sensing phosphatases (VSPs) are homologs of phosphatase and tensin homolog (PTEN), a phosphatidylinositol 3,4-bisphosphate [PI(3,4)P2 ] and phosphatidylinositol 3,4,5-trisphosphate [PI(3,4,5)P3 ] 3-phosphatase. However, VSPs have a wider range of substrates, cleaving 3-phosphate from PI(3,4)P2 and probably PI(3,4,5)P3 as well as 5-phosphate from phosphatidylinositol 4,5-bisphosphate [PI(4,5)P2 ] and PI(3,4,5)P3 in response to membrane depolarization. Recent proposals say these reactions have differing voltage dependence. Using Förster resonance energy transfer probes specific for different PIs in living cells with zebrafish VSP, we quantitate both voltagedependent 5- and 3-phosphatase subreactions against endogenous substrates. These activities become apparent with different voltage thresholds, voltage sensitivities, and catalytic rates. As an analytical tool, we refine a kinetic model that includes the endogenous pools of phosphoinositides, endogenous phosphatase and kinase reactions connecting them, and four exogenous voltage-dependent 5- and 3-phosphatase subreactions of VSP. We show that apparent voltage threshold differences for seeing effects of the 5- and 3-phosphatase activities in cells are not due to different intrinsic voltage dependence of these reactions. Rather, the reactions have a common voltage dependence, and apparent differences arise only because each VSP subreaction has a different absolute catalytic rate that begins to surpass the respective endogenous enzyme activities at different voltages. For zebrafish VSP, our modeling revealed that 3-phosphatase activity against PI(3,4,5)P3 is 55-fold slower than 5-phosphatase activity against PI(4,5)P2 ; thus, PI(4,5)P2 generated more slowly from dephosphorylating PI(3,4,5)P3 might never accumulate. When 5-phosphatase activity was counteracted by coexpression of a phosphatidylinositol 4-phosphate 5-kinase, there was accumulation of PI(4,5)P2 in parallel to PI(3,4,5)P3 dephosphorylation, emphasizing that VSPs can cleave the 3-phosphate of PI(3,4,5)P3 .

Original languageEnglish
Pages (from-to)E3686-E3695
JournalProceedings of the National Academy of Sciences of the United States of America
Volume113
Issue number26
DOIs
StatePublished - 28 Jun 2016

Bibliographical note

Funding Information:
We thank Eamonn J. Dickson, Seung-Ryoung Jung, Mario G. Rosasco, Daewon Moon, and Oscar Vivas for comments on the manuscript, and Lea M. Miller for technical help. This study was supported by the National Institute of Neurological Disorders and Stroke of the NIH (Grant R37NS008174), the Wayne E. Crill Endowed Professorship, the Korean Brain Research Institute basic research program funded by the Ministry of Science, Information and Communications Technology and Future Planning (Grant 2231-415), and the Daegu Gyeongbuk Institute of Science and Technology (DGIST) RandD Program of the Ministry of Science, Information and Communications Technology and Future Planning (Grant 16-BD-06). The Virtual Cell is supported by NIH Grant P41GM103313 from the National Institute of General Medical Sciences.

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