Phosphocholine-Modified Magnetic Nanoparticles for Isolation of C-Reactive Protein from Human Serum

Eunjoo Kim; Se Geun Lee; Hyun Chul Kim; Sung Jun Lee; Chul Su Baek; Eun Sook Choi; Sang Won Jeong

doi:10.1080/01496395.2013.807516

Phosphocholine-Modified Magnetic Nanoparticles for Isolation of C-Reactive Protein from Human Serum

Eunjoo Kim, Se Geun Lee, Hyun Chul Kim, Sung Jun Lee, Chul Su Baek, Eun Sook Choi, Sang Won Jeong

Daegu Gyeongbuk Institute of Science and Technology

Research output: Contribution to journal › Article › peer-review

7 Scopus citations

Abstract

In the present study, we describe the easy isolation of C-reactive protein (CRP) from human serum using phosphocholine-modified magnetic nanoparticles (MNPs). A phosphocholine-based monomer, 3-(4)-vinylbenzyl-12-phosphorylcholine dodecanoate (VPC), was polymerized on methacrylate-coated MNPs and the resulting MNPs (VPC-MNPs) were assessed for their ability to isolate CRP from a human serum sample. CRP could be isolated from human serum with one adsorption step to VPC-MNPs within 1 h. The high purity of the isolated CRP fraction determined by SDS-PAGE indicates a good selectivity of VPC-MNPs for CRP binding. These results demonstrate the feasibility of using ligand-functionalized MNPs for rapid, easy, and efficient protein isolation.

Original language	English
Pages (from-to)	2600-2607
Number of pages	8
Journal	Separation Science and Technology (Philadelphia)
Volume	48
Issue number	17
DOIs	https://doi.org/10.1080/01496395.2013.807516
State	Published - Nov 2013

Bibliographical note

Funding Information:
This work was supported by the DGIST R&D Program (11-NB-01) of the Ministry of Education, Science and Technology of Korea.

Keywords

C-reactive protein (crp)
affinity isolation
magnetic nanoparticle
phosphocholine
protein purification

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title = "Phosphocholine-Modified Magnetic Nanoparticles for Isolation of C-Reactive Protein from Human Serum",

abstract = "In the present study, we describe the easy isolation of C-reactive protein (CRP) from human serum using phosphocholine-modified magnetic nanoparticles (MNPs). A phosphocholine-based monomer, 3-(4)-vinylbenzyl-12-phosphorylcholine dodecanoate (VPC), was polymerized on methacrylate-coated MNPs and the resulting MNPs (VPC-MNPs) were assessed for their ability to isolate CRP from a human serum sample. CRP could be isolated from human serum with one adsorption step to VPC-MNPs within 1 h. The high purity of the isolated CRP fraction determined by SDS-PAGE indicates a good selectivity of VPC-MNPs for CRP binding. These results demonstrate the feasibility of using ligand-functionalized MNPs for rapid, easy, and efficient protein isolation.",

keywords = "C-reactive protein (crp), affinity isolation, magnetic nanoparticle, phosphocholine, protein purification",

author = "Eunjoo Kim and Lee, {Se Geun} and Kim, {Hyun Chul} and Lee, {Sung Jun} and Baek, {Chul Su} and Choi, {Eun Sook} and Jeong, {Sang Won}",

note = "Funding Information: This work was supported by the DGIST R&D Program (11-NB-01) of the Ministry of Education, Science and Technology of Korea.",

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doi = "10.1080/01496395.2013.807516",

language = "English",

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T1 - Phosphocholine-Modified Magnetic Nanoparticles for Isolation of C-Reactive Protein from Human Serum

AU - Kim, Eunjoo

AU - Lee, Se Geun

AU - Kim, Hyun Chul

AU - Lee, Sung Jun

AU - Baek, Chul Su

AU - Choi, Eun Sook

AU - Jeong, Sang Won

N1 - Funding Information: This work was supported by the DGIST R&D Program (11-NB-01) of the Ministry of Education, Science and Technology of Korea.

PY - 2013/11

Y1 - 2013/11

N2 - In the present study, we describe the easy isolation of C-reactive protein (CRP) from human serum using phosphocholine-modified magnetic nanoparticles (MNPs). A phosphocholine-based monomer, 3-(4)-vinylbenzyl-12-phosphorylcholine dodecanoate (VPC), was polymerized on methacrylate-coated MNPs and the resulting MNPs (VPC-MNPs) were assessed for their ability to isolate CRP from a human serum sample. CRP could be isolated from human serum with one adsorption step to VPC-MNPs within 1 h. The high purity of the isolated CRP fraction determined by SDS-PAGE indicates a good selectivity of VPC-MNPs for CRP binding. These results demonstrate the feasibility of using ligand-functionalized MNPs for rapid, easy, and efficient protein isolation.

AB - In the present study, we describe the easy isolation of C-reactive protein (CRP) from human serum using phosphocholine-modified magnetic nanoparticles (MNPs). A phosphocholine-based monomer, 3-(4)-vinylbenzyl-12-phosphorylcholine dodecanoate (VPC), was polymerized on methacrylate-coated MNPs and the resulting MNPs (VPC-MNPs) were assessed for their ability to isolate CRP from a human serum sample. CRP could be isolated from human serum with one adsorption step to VPC-MNPs within 1 h. The high purity of the isolated CRP fraction determined by SDS-PAGE indicates a good selectivity of VPC-MNPs for CRP binding. These results demonstrate the feasibility of using ligand-functionalized MNPs for rapid, easy, and efficient protein isolation.

KW - C-reactive protein (crp)

KW - affinity isolation

KW - magnetic nanoparticle

KW - phosphocholine

KW - protein purification

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DO - 10.1080/01496395.2013.807516

M3 - Article

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SN - 0149-6395

VL - 48

SP - 2600

EP - 2607

JO - Separation Science and Technology (Philadelphia)

JF - Separation Science and Technology (Philadelphia)

IS - 17

ER -

Phosphocholine-Modified Magnetic Nanoparticles for Isolation of C-Reactive Protein from Human Serum

Abstract

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Keywords

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