Overexpression and unique rearrangement of VH2 transcripts in immunoglobulin variable heavy chain genes in ankylosing spondylitis patients

Yeon Joo Kim; Nayoung Kim; Min Kyung Lee; Hyo Jin Choi; Han Joo Baek; Chang Hoon Nam

doi:10.1007/978-3-642-17913-6_14

Overexpression and unique rearrangement of VH2 transcripts in immunoglobulin variable heavy chain genes in ankylosing spondylitis patients

Yeon Joo Kim, Nayoung Kim, Min Kyung Lee, Hyo Jin Choi, Han Joo Baek, Chang Hoon Nam

Department of New Biology

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

Abstract

To identify immunoglobulin (Ig) variable heavy chain (VH) gene usages in Korean ankylosing spondylitis (AS) patients, expression level of VH2 genes from peripheral blood mononuclear cells (PBMCs) of 8 AS patients and 9 healthy donors was analysed by quantitative real-time PCR (Q-PCR). Q-PCR results demonstrated VH2 genes were overexpressed in AS patients (Relative amount of mRNA of VH2 genes to a house¬keeping gene, 7.13±7.77 vs, 0.68±0.55; p<0.0001). The sequence analysis revealed the majority of them contained CDC42 binding protein kinase beta (CDC42 BPB) genes. The insertion of CDC42 BPB gene was confirmed by PCR with primers corresponding CDC42 BPB and CH genes. Our study revealed VH2 overexpression and unique rearrangement in Ig VH genes from peripheral blood of AS patients. This may imply aberrant immunoglobulin gene rearrangement in B cell occurs in Korean AS patients, which requires further investigation.

Original language	English
Title of host publication	EKC 2010 - Proceedings of the EU-Korea Conference on Science and Technology
Editors	Jehyun Lee, Man-Wook Han
Publisher	Springer Science and Business Media, LLC
Pages	113-122
Number of pages	10
ISBN (Print)	9783642179129
DOIs	https://doi.org/10.1007/978-3-642-17913-6_14
State	Published - 2011
Event	3rd EU Korea Conference on Science and Technology, EKC 2010 - Vienna, Austria Duration: 29 Jul 2010 → 31 Jul 2010

Publication series

Name	Springer Proceedings in Physics
Volume	138
ISSN (Print)	0930-8989
ISSN (Electronic)	1867-4941

Conference

Conference	3rd EU Korea Conference on Science and Technology, EKC 2010
Country/Territory	Austria
City	Vienna
Period	29/07/10 → 31/07/10

Bibliographical note

Publisher Copyright:
© Springer-Verlag Berlin Heidelberg 2011.

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10.1007/978-3-642-17913-6_14

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Kim, Y. J., Kim, N., Lee, M. K., Choi, H. J., Baek, H. J., & Nam, C. H. (2011). Overexpression and unique rearrangement of VH2 transcripts in immunoglobulin variable heavy chain genes in ankylosing spondylitis patients. In J. Lee, & M.-W. Han (Eds.), EKC 2010 - Proceedings of the EU-Korea Conference on Science and Technology (pp. 113-122). (Springer Proceedings in Physics; Vol. 138). Springer Science and Business Media, LLC. https://doi.org/10.1007/978-3-642-17913-6_14

Kim, Yeon Joo ; Kim, Nayoung ; Lee, Min Kyung et al. / Overexpression and unique rearrangement of VH2 transcripts in immunoglobulin variable heavy chain genes in ankylosing spondylitis patients. EKC 2010 - Proceedings of the EU-Korea Conference on Science and Technology. editor / Jehyun Lee ; Man-Wook Han. Springer Science and Business Media, LLC, 2011. pp. 113-122 (Springer Proceedings in Physics).

@inproceedings{3931e8c61d62418eaeb1b87ac99d2432,

title = "Overexpression and unique rearrangement of VH2 transcripts in immunoglobulin variable heavy chain genes in ankylosing spondylitis patients",

abstract = "To identify immunoglobulin (Ig) variable heavy chain (VH) gene usages in Korean ankylosing spondylitis (AS) patients, expression level of VH2 genes from peripheral blood mononuclear cells (PBMCs) of 8 AS patients and 9 healthy donors was analysed by quantitative real-time PCR (Q-PCR). Q-PCR results demonstrated VH2 genes were overexpressed in AS patients (Relative amount of mRNA of VH2 genes to a house¬keeping gene, 7.13±7.77 vs, 0.68±0.55; p<0.0001). The sequence analysis revealed the majority of them contained CDC42 binding protein kinase beta (CDC42 BPB) genes. The insertion of CDC42 BPB gene was confirmed by PCR with primers corresponding CDC42 BPB and CH genes. Our study revealed VH2 overexpression and unique rearrangement in Ig VH genes from peripheral blood of AS patients. This may imply aberrant immunoglobulin gene rearrangement in B cell occurs in Korean AS patients, which requires further investigation.",

author = "Kim, {Yeon Joo} and Nayoung Kim and Lee, {Min Kyung} and Choi, {Hyo Jin} and Baek, {Han Joo} and Nam, {Chang Hoon}",

note = "Publisher Copyright: {\textcopyright} Springer-Verlag Berlin Heidelberg 2011.; 3rd EU Korea Conference on Science and Technology, EKC 2010 ; Conference date: 29-07-2010 Through 31-07-2010",

year = "2011",

doi = "10.1007/978-3-642-17913-6_14",

language = "English",

isbn = "9783642179129",

series = "Springer Proceedings in Physics",

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}

Kim, YJ, Kim, N, Lee, MK, Choi, HJ, Baek, HJ & Nam, CH 2011, Overexpression and unique rearrangement of VH2 transcripts in immunoglobulin variable heavy chain genes in ankylosing spondylitis patients. in J Lee & M-W Han (eds), EKC 2010 - Proceedings of the EU-Korea Conference on Science and Technology. Springer Proceedings in Physics, vol. 138, Springer Science and Business Media, LLC, pp. 113-122, 3rd EU Korea Conference on Science and Technology, EKC 2010, Vienna, Austria, 29/07/10. https://doi.org/10.1007/978-3-642-17913-6_14

Overexpression and unique rearrangement of VH2 transcripts in immunoglobulin variable heavy chain genes in ankylosing spondylitis patients. / Kim, Yeon Joo; Kim, Nayoung; Lee, Min Kyung et al.
EKC 2010 - Proceedings of the EU-Korea Conference on Science and Technology. ed. / Jehyun Lee; Man-Wook Han. Springer Science and Business Media, LLC, 2011. p. 113-122 (Springer Proceedings in Physics; Vol. 138).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

TY - GEN

T1 - Overexpression and unique rearrangement of VH2 transcripts in immunoglobulin variable heavy chain genes in ankylosing spondylitis patients

AU - Kim, Yeon Joo

AU - Kim, Nayoung

AU - Lee, Min Kyung

AU - Choi, Hyo Jin

AU - Baek, Han Joo

AU - Nam, Chang Hoon

PY - 2011

Y1 - 2011

N2 - To identify immunoglobulin (Ig) variable heavy chain (VH) gene usages in Korean ankylosing spondylitis (AS) patients, expression level of VH2 genes from peripheral blood mononuclear cells (PBMCs) of 8 AS patients and 9 healthy donors was analysed by quantitative real-time PCR (Q-PCR). Q-PCR results demonstrated VH2 genes were overexpressed in AS patients (Relative amount of mRNA of VH2 genes to a house¬keeping gene, 7.13±7.77 vs, 0.68±0.55; p<0.0001). The sequence analysis revealed the majority of them contained CDC42 binding protein kinase beta (CDC42 BPB) genes. The insertion of CDC42 BPB gene was confirmed by PCR with primers corresponding CDC42 BPB and CH genes. Our study revealed VH2 overexpression and unique rearrangement in Ig VH genes from peripheral blood of AS patients. This may imply aberrant immunoglobulin gene rearrangement in B cell occurs in Korean AS patients, which requires further investigation.

AB - To identify immunoglobulin (Ig) variable heavy chain (VH) gene usages in Korean ankylosing spondylitis (AS) patients, expression level of VH2 genes from peripheral blood mononuclear cells (PBMCs) of 8 AS patients and 9 healthy donors was analysed by quantitative real-time PCR (Q-PCR). Q-PCR results demonstrated VH2 genes were overexpressed in AS patients (Relative amount of mRNA of VH2 genes to a house¬keeping gene, 7.13±7.77 vs, 0.68±0.55; p<0.0001). The sequence analysis revealed the majority of them contained CDC42 binding protein kinase beta (CDC42 BPB) genes. The insertion of CDC42 BPB gene was confirmed by PCR with primers corresponding CDC42 BPB and CH genes. Our study revealed VH2 overexpression and unique rearrangement in Ig VH genes from peripheral blood of AS patients. This may imply aberrant immunoglobulin gene rearrangement in B cell occurs in Korean AS patients, which requires further investigation.

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SN - 9783642179129

T3 - Springer Proceedings in Physics

SP - 113

EP - 122

BT - EKC 2010 - Proceedings of the EU-Korea Conference on Science and Technology

A2 - Lee, Jehyun

A2 - Han, Man-Wook

PB - Springer Science and Business Media, LLC

T2 - 3rd EU Korea Conference on Science and Technology, EKC 2010

Y2 - 29 July 2010 through 31 July 2010

ER -

Kim YJ, Kim N, Lee MK, Choi HJ, Baek HJ, Nam CH. Overexpression and unique rearrangement of VH2 transcripts in immunoglobulin variable heavy chain genes in ankylosing spondylitis patients. In Lee J, Han MW, editors, EKC 2010 - Proceedings of the EU-Korea Conference on Science and Technology. Springer Science and Business Media, LLC. 2011. p. 113-122. (Springer Proceedings in Physics). doi: 10.1007/978-3-642-17913-6_14

Overexpression and unique rearrangement of VH2 transcripts in immunoglobulin variable heavy chain genes in ankylosing spondylitis patients

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