Non-contact High-Frequency Ultrasound Microbeam Stimulation for Studying Mechanotransduction in Human Umbilical Vein Endothelial Cells

Jae Youn Hwang; Hae Gyun Lim; Chi Woo Yoon; Kwok Ho Lam; Sangpil Yoon; Changyang Lee; Chi Tat Chiu; Bong Jin Kang; Hyung Ham Kim; K. Kirk Shung

doi:10.1016/j.ultrasmedbio.2014.03.018

Non-contact High-Frequency Ultrasound Microbeam Stimulation for Studying Mechanotransduction in Human Umbilical Vein Endothelial Cells

Jae Youn Hwang, Hae Gyun Lim, Chi Woo Yoon, Kwok Ho Lam, Sangpil Yoon, Changyang Lee, Chi Tat Chiu, Bong Jin Kang, Hyung Ham Kim, K. Kirk Shung

Department of Electrical Engineering and Computer Science

Research output: Contribution to journal › Article › peer-review

32 Scopus citations

Abstract

We describe how contactless high-frequency ultrasound microbeam stimulation (HFUMS) is capable of eliciting cytoplasmic calcium (Ca²⁺) elevation in human umbilical vein endothelial cells. The cellular mechanotransduction process, which includes cell sensing and adaptation to the mechanical micro-environment, has been studied extensively in recent years. A variety of tools for mechanical stimulation have been developed to produce cellular responses. We developed a novel tool, a highly focused ultrasound microbeam, for non-contact cell stimulation at a microscale. This tool, at 200 MHz, was applied to human umbilical vein endothelial cells to investigate its potential to elicit an elevation in cytoplasmic Ca²⁺ levels. It was found that the response was dose dependent, and moreover, extracellular Ca²⁺ and cytoplasmic Ca²⁺ stores were involved in the Ca²⁺ elevation. These results suggest that high-frequency ultrasound microbeam stimulation is potentially a novel non-contact tool for studying cellular mechanotransduction if the acoustic pressures at such high frequencies can be quantified.

Original language	English
Pages (from-to)	2172-2182
Number of pages	11
Journal	Ultrasound in Medicine and Biology
Volume	40
Issue number	9
DOIs	https://doi.org/10.1016/j.ultrasmedbio.2014.03.018
State	Published - Sep 2014

Bibliographical note

Funding Information:
This work has been supported by the National Institutes of Health Grants, United States of America ( R01-EB012058 and P41-EB002182) and International Collaborative R&D Program funded by the Ministry of Trade, Industry & Energy (MOTIE), Korea (N01150049, Developing high frequency bandwidth [40∼60 MHz] high resolution image system and probe technology for diagnosing cardiovascular lesion) to K. K. Shung and MIREBraiN Start-up Program by the Daegu Gyeongbuk Institute of Science & Technology to J.Y. Hwang.

Keywords

Calcium fluorescence imaging
High-frequency ultrasound microbeam
Human umbilical vein endothelial cells
Mechanotransduction

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10.1016/j.ultrasmedbio.2014.03.018

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Hwang, J. Y., Lim, H. G., Yoon, C. W., Lam, K. H., Yoon, S., Lee, C., Chiu, C. T., Kang, B. J., Kim, H. H., & Shung, K. K. (2014). Non-contact High-Frequency Ultrasound Microbeam Stimulation for Studying Mechanotransduction in Human Umbilical Vein Endothelial Cells. Ultrasound in Medicine and Biology, 40(9), 2172-2182. https://doi.org/10.1016/j.ultrasmedbio.2014.03.018

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title = "Non-contact High-Frequency Ultrasound Microbeam Stimulation for Studying Mechanotransduction in Human Umbilical Vein Endothelial Cells",

abstract = "We describe how contactless high-frequency ultrasound microbeam stimulation (HFUMS) is capable of eliciting cytoplasmic calcium (Ca2+) elevation in human umbilical vein endothelial cells. The cellular mechanotransduction process, which includes cell sensing and adaptation to the mechanical micro-environment, has been studied extensively in recent years. A variety of tools for mechanical stimulation have been developed to produce cellular responses. We developed a novel tool, a highly focused ultrasound microbeam, for non-contact cell stimulation at a microscale. This tool, at 200 MHz, was applied to human umbilical vein endothelial cells to investigate its potential to elicit an elevation in cytoplasmic Ca2+ levels. It was found that the response was dose dependent, and moreover, extracellular Ca2+ and cytoplasmic Ca2+ stores were involved in the Ca2+ elevation. These results suggest that high-frequency ultrasound microbeam stimulation is potentially a novel non-contact tool for studying cellular mechanotransduction if the acoustic pressures at such high frequencies can be quantified.",

keywords = "Calcium fluorescence imaging, High-frequency ultrasound microbeam, Human umbilical vein endothelial cells, Mechanotransduction",

author = "Hwang, {Jae Youn} and Lim, {Hae Gyun} and Yoon, {Chi Woo} and Lam, {Kwok Ho} and Sangpil Yoon and Changyang Lee and Chiu, {Chi Tat} and Kang, {Bong Jin} and Kim, {Hyung Ham} and Shung, {K. Kirk}",

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AU - Kang, Bong Jin

AU - Kim, Hyung Ham

AU - Shung, K. Kirk

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N2 - We describe how contactless high-frequency ultrasound microbeam stimulation (HFUMS) is capable of eliciting cytoplasmic calcium (Ca2+) elevation in human umbilical vein endothelial cells. The cellular mechanotransduction process, which includes cell sensing and adaptation to the mechanical micro-environment, has been studied extensively in recent years. A variety of tools for mechanical stimulation have been developed to produce cellular responses. We developed a novel tool, a highly focused ultrasound microbeam, for non-contact cell stimulation at a microscale. This tool, at 200 MHz, was applied to human umbilical vein endothelial cells to investigate its potential to elicit an elevation in cytoplasmic Ca2+ levels. It was found that the response was dose dependent, and moreover, extracellular Ca2+ and cytoplasmic Ca2+ stores were involved in the Ca2+ elevation. These results suggest that high-frequency ultrasound microbeam stimulation is potentially a novel non-contact tool for studying cellular mechanotransduction if the acoustic pressures at such high frequencies can be quantified.

AB - We describe how contactless high-frequency ultrasound microbeam stimulation (HFUMS) is capable of eliciting cytoplasmic calcium (Ca2+) elevation in human umbilical vein endothelial cells. The cellular mechanotransduction process, which includes cell sensing and adaptation to the mechanical micro-environment, has been studied extensively in recent years. A variety of tools for mechanical stimulation have been developed to produce cellular responses. We developed a novel tool, a highly focused ultrasound microbeam, for non-contact cell stimulation at a microscale. This tool, at 200 MHz, was applied to human umbilical vein endothelial cells to investigate its potential to elicit an elevation in cytoplasmic Ca2+ levels. It was found that the response was dose dependent, and moreover, extracellular Ca2+ and cytoplasmic Ca2+ stores were involved in the Ca2+ elevation. These results suggest that high-frequency ultrasound microbeam stimulation is potentially a novel non-contact tool for studying cellular mechanotransduction if the acoustic pressures at such high frequencies can be quantified.

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Non-contact High-Frequency Ultrasound Microbeam Stimulation for Studying Mechanotransduction in Human Umbilical Vein Endothelial Cells

Abstract

Bibliographical note

Keywords

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