Abstract
A simple in vitro model for human organ-to-organ interaction studies was realized by using spherical microtissues (MTs) cultured in a 96-well-format-based microfluidic platform. The model was validated by comparing the bio-activation effects of an anti-cancer pro-drug, cyclophosphamide (CPA), under static and perfusion conditions in a liver-tumor network. Primary human liver (hLi) MTs and human colon carcinoma (HCT116) MTs were fluidically interconnected for realizing a liver-tumor model in a physiological environment. Medium was perfused through microfluidic channels without tubing and pumps by using a gravity-driven flow concept.
| Original language | English |
|---|---|
| Title of host publication | MicroTAS 2015 - 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences |
| Publisher | Chemical and Biological Microsystems Society |
| Pages | 747-749 |
| Number of pages | 3 |
| ISBN (Electronic) | 9780979806483 |
| State | Published - 2015 |
| Event | 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2015 - Gyeongju, Korea, Republic of Duration: 25 Oct 2015 → 29 Oct 2015 |
Publication series
| Name | MicroTAS 2015 - 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences |
|---|
Conference
| Conference | 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2015 |
|---|---|
| Country/Territory | Korea, Republic of |
| City | Gyeongju |
| Period | 25/10/15 → 29/10/15 |
Bibliographical note
Publisher Copyright:© 15CBMS-0001.
Keywords
- 3D cell culture
- Human liver-tumor network
- Microfluidics
- Microtissue
- Spheroid
