Multimode optical imaging of small animals: Development and applications

J. Y. Hwang, C. Moffatt-Blue, O. Equils, M. Fujita, J. Jeong, N. M. Khazenzon, E. Lindsley, J. Ljubimova, A. G. Nowatzyk, D. L. Farkas, S. Wachsmann-Hogiu

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

9 Scopus citations

Abstract

We present an optical system for small animal imaging that can combine various in vivo imaging modalities, including fluorescence (intensity and lifetime), spectral, and trans-illumination imaging. This system consists of light-tight box with ultrafast pulsed or cw laser light excitation, motorized translational and rotational stages, a telecentric lens for detection, and a cooled CCD camera that can be coupled to an ultrafast time-gated intensifier. All components are modular, making possible laser excitation at various wavelengths and pulse lengths, and signal detection in a variety of ways (multimode). Results of drug nanoconjugate carrier delivery studies in mice are presented. Conventional and spectrally-resolved fluorescence images reveal details of in vivo drug nanoconjugate carrier accumulation within the tumor region and several organs in real time. By multi-spectral image analysis of ex vivo specimens from the same mice, we were able to evaluate the extent and topology of drug nanoconjugate carrier distribution into specific organs and the tumor itself.

Original languageEnglish
Title of host publicationImaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues V
DOIs
StatePublished - 2007
EventImaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues V - San Jose, CA, United States
Duration: 22 Jan 200724 Jan 2007

Publication series

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume6441
ISSN (Print)1605-7422

Conference

ConferenceImaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues V
Country/TerritoryUnited States
CitySan Jose, CA
Period22/01/0724/01/07

Keywords

  • Bioluminescence imaging
  • Fluorescence imaging
  • Fluorescence lifetime imaging
  • Hyperspectral imaging and analysis
  • Mouse model
  • Multi-mode imaging system
  • Nanoconstruct
  • Trans-illumination imaging

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