TY - JOUR
T1 - MPK9 and MPK12 function in SA-induced stomatal closure in arabidopsis thaliana
AU - Khokon, Md Atiqur Rahman
AU - Salam, Mohammad Abdus
AU - Jammes, Fabien
AU - Ye, Wenxiu
AU - Hossain, Mohammad Anowar
AU - Okuma, Eiji
AU - Nakamura, Yoshimasa
AU - Mori, Izumi C.
AU - Kwak, June M.
AU - Murata, Yoshiyuki
N1 - Publisher Copyright:
© 2017 Japan Society for Bioscience, Biotechnology, and Agrochemistry
PY - 2017
Y1 - 2017
N2 - Salicylic acid (SA) induces stomatal closure sharing several components with abscisic acid (ABA) and methyl jasmonate (MeJA) signaling. We have previously shown that two guard cell-preferential mitogen-activated protein kinases (MAPKs), MPK9 and MPK12, positively regulate ABA signaling and MeJA signaling in Arabidopsis thaliana. In this study, we examined whether these two MAPKs are involved in SA-induced stomatal closure using genetic mutants and a pharmacological, MAPKK inhibitor. Salicylic acid induced stomatal closure in mpk9 and mpk12 single mutants but not in mpk9 mpk12 double mutants. The MAPKK inhibitor PD98059 inhibited SA-induced stomatal closure in wild-type plants. Salicylic acid induced extracellular reactive oxygen species (ROS) production, intracellular ROS accumulation, and cytosolic alkalization in the mpk9, mpk12, and mpk9 mpk12 mutants. Moreover, SA-activated S-type anion channels in guard cells of wild-type plants but not in guard cells of mpk9 mpk12 double mutants. These results imply that MPK9 and MPK12 are positive regulators of SA signaling in Arabidopsis guard cells.
AB - Salicylic acid (SA) induces stomatal closure sharing several components with abscisic acid (ABA) and methyl jasmonate (MeJA) signaling. We have previously shown that two guard cell-preferential mitogen-activated protein kinases (MAPKs), MPK9 and MPK12, positively regulate ABA signaling and MeJA signaling in Arabidopsis thaliana. In this study, we examined whether these two MAPKs are involved in SA-induced stomatal closure using genetic mutants and a pharmacological, MAPKK inhibitor. Salicylic acid induced stomatal closure in mpk9 and mpk12 single mutants but not in mpk9 mpk12 double mutants. The MAPKK inhibitor PD98059 inhibited SA-induced stomatal closure in wild-type plants. Salicylic acid induced extracellular reactive oxygen species (ROS) production, intracellular ROS accumulation, and cytosolic alkalization in the mpk9, mpk12, and mpk9 mpk12 mutants. Moreover, SA-activated S-type anion channels in guard cells of wild-type plants but not in guard cells of mpk9 mpk12 double mutants. These results imply that MPK9 and MPK12 are positive regulators of SA signaling in Arabidopsis guard cells.
KW - Cytosolic alkalization
KW - Mitogen-activated protein kinase
KW - Reactive oxygen species
KW - S-type anion channel in guard cells
KW - Salicylic acid
UR - https://www.scopus.com/pages/publications/85021175779
U2 - 10.1080/09168451.2017.1308244
DO - 10.1080/09168451.2017.1308244
M3 - Article
C2 - 28387156
AN - SCOPUS:85021175779
SN - 0916-8451
VL - 81
SP - 1394
EP - 1400
JO - Bioscience, Biotechnology and Biochemistry
JF - Bioscience, Biotechnology and Biochemistry
IS - 7
ER -