Microfabricated embryonic stem cell divider for large-scale propagation of human embryonic stem cells

Minseok S. Kim; Janghwan Kim; Hyo Won Han; Yee Sook Cho; Yong Mahn Han; Je Kyun Park

doi:10.1039/b617760n

Microfabricated embryonic stem cell divider for large-scale propagation of human embryonic stem cells

Minseok S. Kim, Janghwan Kim, Hyo Won Han, Yee Sook Cho, Yong Mahn Han, Je Kyun Park

Department of New Biology

Research output: Contribution to journal › Article › peer-review

17 Scopus citations

Abstract

We have developed a novel method for fabricating an embryonic stem cell divider (ESCD) constructed from a poly(dimethylsiloxane) (PDMS) replica with a square or hexagonal pattern, and have proposed a new dissociation method for human embryonic stem cells (ESCs). An aspect ratio of the device as high as 2 was perfectly replicated in the cutting line. Using the ESCD, human ESC colonies can be easily and efficiently dissociated into regular-sized ESC clumps without enzymatic treatment. The regularity of the ESC clumps dissociated by the ESCD was compared to that dissociated by a conventional mechanical method. Its quality and reliability were confirmed by maintaining undifferentiated ESCs up to the 15th passage. The ESCD will contribute to the advance quality control of in vitro ESC cultures and allow large-scale production of qualified ESCs with tremendous time- and work-saving.

Original language	English
Pages (from-to)	513-515
Number of pages	3
Journal	Lab on a Chip
Volume	7
Issue number	4
DOIs	https://doi.org/10.1039/b617760n
State	Published - 2007

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title = "Microfabricated embryonic stem cell divider for large-scale propagation of human embryonic stem cells",

abstract = "We have developed a novel method for fabricating an embryonic stem cell divider (ESCD) constructed from a poly(dimethylsiloxane) (PDMS) replica with a square or hexagonal pattern, and have proposed a new dissociation method for human embryonic stem cells (ESCs). An aspect ratio of the device as high as 2 was perfectly replicated in the cutting line. Using the ESCD, human ESC colonies can be easily and efficiently dissociated into regular-sized ESC clumps without enzymatic treatment. The regularity of the ESC clumps dissociated by the ESCD was compared to that dissociated by a conventional mechanical method. Its quality and reliability were confirmed by maintaining undifferentiated ESCs up to the 15th passage. The ESCD will contribute to the advance quality control of in vitro ESC cultures and allow large-scale production of qualified ESCs with tremendous time- and work-saving.",

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doi = "10.1039/b617760n",

language = "English",

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T1 - Microfabricated embryonic stem cell divider for large-scale propagation of human embryonic stem cells

AU - Kim, Minseok S.

AU - Kim, Janghwan

AU - Han, Hyo Won

AU - Cho, Yee Sook

AU - Han, Yong Mahn

AU - Park, Je Kyun

PY - 2007

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N2 - We have developed a novel method for fabricating an embryonic stem cell divider (ESCD) constructed from a poly(dimethylsiloxane) (PDMS) replica with a square or hexagonal pattern, and have proposed a new dissociation method for human embryonic stem cells (ESCs). An aspect ratio of the device as high as 2 was perfectly replicated in the cutting line. Using the ESCD, human ESC colonies can be easily and efficiently dissociated into regular-sized ESC clumps without enzymatic treatment. The regularity of the ESC clumps dissociated by the ESCD was compared to that dissociated by a conventional mechanical method. Its quality and reliability were confirmed by maintaining undifferentiated ESCs up to the 15th passage. The ESCD will contribute to the advance quality control of in vitro ESC cultures and allow large-scale production of qualified ESCs with tremendous time- and work-saving.

AB - We have developed a novel method for fabricating an embryonic stem cell divider (ESCD) constructed from a poly(dimethylsiloxane) (PDMS) replica with a square or hexagonal pattern, and have proposed a new dissociation method for human embryonic stem cells (ESCs). An aspect ratio of the device as high as 2 was perfectly replicated in the cutting line. Using the ESCD, human ESC colonies can be easily and efficiently dissociated into regular-sized ESC clumps without enzymatic treatment. The regularity of the ESC clumps dissociated by the ESCD was compared to that dissociated by a conventional mechanical method. Its quality and reliability were confirmed by maintaining undifferentiated ESCs up to the 15th passage. The ESCD will contribute to the advance quality control of in vitro ESC cultures and allow large-scale production of qualified ESCs with tremendous time- and work-saving.

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Microfabricated embryonic stem cell divider for large-scale propagation of human embryonic stem cells

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