Mag-centrifugal microfluidic separation for rapid purification of dorsal root ganglion neurons

Hee Jae Lee, Ji Hoon Kim, Woon Hae Kim, Hyun Young Shin, Seung Joon Lee, Joseph Sunoo, Yun Jeong Mo, Yu Seon Kim, Yun Il Lee, Minseok S. Kim

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

Abstract

Dorsal root ganglion (DRG) neurons have been widely used as a good model for sensory neurons of the peripheral nervous system in neurology. However, purification process is essential to only acquire the sensory neurons since the DRG organ is composed of several types of cells including fibroblast, Schwann and epithelial cells. In this study, we introduce a novel hybrid isolation method combining centrifugal and immunomagnetic separations to selectively remove the Schwann cells, fibroblasts and epithelial cells with fast manner. As a result, we obtained high purity and high yield of primary sensory neurons and achieved a reliable and rapid purification method through an automated process.

Original languageEnglish
Title of host publication22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018
PublisherChemical and Biological Microsystems Society
Pages2323-2324
Number of pages2
ISBN (Electronic)9781510897571
StatePublished - 2018
Event22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018 - Kaohsiung, Taiwan, Province of China
Duration: 11 Nov 201815 Nov 2018

Publication series

Name22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018
Volume4

Conference

Conference22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018
Country/TerritoryTaiwan, Province of China
CityKaohsiung
Period11/11/1815/11/18

Bibliographical note

Publisher Copyright:
Copyright © (2018) by Chemical and Biological Microsystems Society. All rights reserved.

Keywords

  • Cell purification
  • Centrifugal microfluidics
  • DRG neuron
  • Immunomagnetic separation

Fingerprint

Dive into the research topics of 'Mag-centrifugal microfluidic separation for rapid purification of dorsal root ganglion neurons'. Together they form a unique fingerprint.

Cite this