Abstract
Dorsal root ganglion (DRG) neurons have been widely used as a good model for sensory neurons of the peripheral nervous system in neurology. However, purification process is essential to only acquire the sensory neurons since the DRG organ is composed of several types of cells including fibroblast, Schwann and epithelial cells. In this study, we introduce a novel hybrid isolation method combining centrifugal and immunomagnetic separations to selectively remove the Schwann cells, fibroblasts and epithelial cells with fast manner. As a result, we obtained high purity and high yield of primary sensory neurons and achieved a reliable and rapid purification method through an automated process.
Original language | English |
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Title of host publication | 22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018 |
Publisher | Chemical and Biological Microsystems Society |
Pages | 2323-2324 |
Number of pages | 2 |
ISBN (Electronic) | 9781510897571 |
State | Published - 2018 |
Event | 22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018 - Kaohsiung, Taiwan, Province of China Duration: 11 Nov 2018 → 15 Nov 2018 |
Publication series
Name | 22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018 |
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Volume | 4 |
Conference
Conference | 22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018 |
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Country/Territory | Taiwan, Province of China |
City | Kaohsiung |
Period | 11/11/18 → 15/11/18 |
Bibliographical note
Publisher Copyright:Copyright © (2018) by Chemical and Biological Microsystems Society. All rights reserved.
Keywords
- Cell purification
- Centrifugal microfluidics
- DRG neuron
- Immunomagnetic separation