Abstract
Although accumulating proteomic analyses have supported the fact that mitochondrial oxidative phosphorylation (OXPHOS) complexes are localized in lipid rafts, which mediate cell signaling, immune response and host-pathogen interactions, there has been no in-depth study of the physiological functions of lipid-raft OXPHOS complexes. Here, we show that many subunits of OXPHOS complexes were identified from the lipid rafts of human adipocytes, C2C12 myotubes, Jurkat cells and surface biotin-labeled Jurkat cells via shotgun proteomic analysis. We discuss the findings of OXPHOS complexes in lipid rafts, the role of the surface ATP synthase complex as a receptor for various ligands and extracellular superoxide generation by plasma membrane oxidative phosphorylation complexes.
Original language | English |
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Pages (from-to) | 849-866 |
Number of pages | 18 |
Journal | Expert Review of Proteomics |
Volume | 7 |
Issue number | 6 |
DOIs | |
State | Published - Dec 2010 |
Bibliographical note
Funding Information:This work was supported by grants awarded to Young-Gyu Ko from the Basic Science Program (C00286) and Basic Research Laboratory Program (0001197) of the Korea Research Foundation, and National R&D Program for Cancer Control, Korea Ministry of Health, Welfare and Family Affairs (09201800). The authors have no other relevant affiliations or financial involvement with any organization or entity with a financial interest in or financial conflict with the subject matter or materials discussed in the manuscript apart from those disclosed.
Keywords
- ATP synthase
- detergent-resistant lipid rafts
- extracellular ATP
- extracellular superoxide
- oxidative phosphorylation complexes
- oxygen consumption