Isolation and characterization of two cDNA clones that are rapidly induced during the wound response of Arabidopsis thaliana

Differential screening of a cDNA library of Arabidopsis thaliana constructed from the plant tissues harvested 1 h after wounding resulted in isolation of 2 wound-inducible cDNA clones. Kinetic analysis revealed that the corresponding genes are rapidly induced upon wounding. Expression of these clones reached the maximum level around 1-1.5 h after wounding and then were progressively reduced. The time by which expression returned to the control level was around 3 h after wounding. Partial sequence analysis revealed that the two clones are highly homologous to the S-adenosylmethionine synthetase and the glutathione-S-transferase gene, respectively.