Intracellular substrates of a heme-containing ascorbate oxidase in Pleurotus ostreatus

Seung Rock Lee, Woo Jeong Joo, Yong Un Baek, Youn Kyong Lee, Seong Woon Yu, Yeon Ran Kim, Kee Oh Chay, Seung Hyun Cho, Sa Ouk Rang

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6 Scopus citations

Abstract

A novel heme-containing ascorbate oxidase isolated from oyster mushroom, Pleurotus ostreatus, catalyzes oxidation of ascorbic acid (Kim et al., 1996). In this report, we describe the identification of intracellular substrates of the enzyme in the mushroom. Six compounds, which can serve as substrate of the heme-containing ascorbate oxidase, were identified as L-ascorbic acid, D-erythroascorbic acid, 5-O-(α-D-glucopyranosyl)-D-erythroascorbic acid, 5-O-(α-D-xylopyranosyl)-D-erythroascorbic acid, 5-methyl-5-O-(α-D- gluco-pyranosyl)-D-erythroascorbic acid, and 5-methyl-5-O-(α-D- xylopyranosyl)-D-erythroascorbic acid. All of the compounds were oxidized at a significant rate by the heme-containing ascorbate oxidase. Oxidation of the compounds produced equimolar amounts of hydrogen peroxide per mole of substrate.

Original languageEnglish
Pages (from-to)178-186
Number of pages9
JournalJournal of Microbiology
Volume47
Issue number2
DOIs
StatePublished - Apr 2009

Bibliographical note

Funding Information:
We thank P. B. Chock (NIH, USA) for reading the manuscript. This work was supported by a research grant for SRC (Research Center for Molecular Microbiology, Seoul National University) from the Korea Science and Engineering Foundation (KOSEF). S-R Lee was partly supported by grants KRF-2007-314-e00036 and KRF-2005-070-C00086 from the Korea Research Foundation.

Keywords

  • Ascorbate oxidase
  • Pleurotus ostreatus
  • Substrates

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