Interaction between the keratin cytoskeleton and eEF1Bγ affects protein synthesis in epithelial cells

Seyun Kim, Juliane Kellner, Chang Hun Lee, Pierre A. Coulombe

Research output: Contribution to journalArticlepeer-review

45 Scopus citations

Abstract

Eukaryotic elongation factor-1 (eEF1) is essential for peptide-chain elongation during translation. We report that its γ subunit (eEF1Bγ) specifically binds, and bundles, keratin intermediate filaments. Disrupting this interaction depresses translation by ∼20% and selectively increases 80S ribosomes in epithelial cells, an outcome recapitulated by RNA interference-mediated silencing of eEF1Bγ. These findings extend the emerging relationship between keratin proteins and the translational machinery.

Original languageEnglish
Pages (from-to)982-983
Number of pages2
JournalNature Structural and Molecular Biology
Volume14
Issue number10
DOIs
StatePublished - Oct 2007

Bibliographical note

Funding Information:
We thank J. Lorsch, C. Parent, T. Kinzy, J. Dijk, D. Robinson and Y. Zheng for help. This work was supported by US National Institutes of Health grants AR42047 and AR44232 to P.A.C.

Fingerprint

Dive into the research topics of 'Interaction between the keratin cytoskeleton and eEF1Bγ affects protein synthesis in epithelial cells'. Together they form a unique fingerprint.

Cite this