Intelligent spectral signature bio-imaging in vivo for surgical applications

Jihoon Jeong, Philip K. Frykman, Mark Gaon, Alice P. Chung, Erik H. Lindsley, Jae Y. Hwang, Daniel L. Farkas

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

3 Scopus citations

Abstract

Multi-spectral imaging provides digital images of a scene or object at a large, usually sequential number of wavelengths, generating precise optical spectra at every pixel. We use the term "spectral signature" for a quantitative plot of optical property variations as a function of wavelengths. We present here intelligent spectral signature bio-imaging methods we developed, including automatic signature selection based on machine learning algorithms and database search-based automatic color allocations, and selected visualization schemes matching these approaches, Using this intelligent spectral signature bio-imaging method, we could discriminate normal and aganglionic colon tissue of the Hirschsprung's disease mouse model with over 95% sensitivity and specificity in various similarity measure methods and various anatomic organs such as parathyroid gland, thyroid gland and pre-tracheal fat in dissected neck of the rat in vivo.

Original languageEnglish
Title of host publicationImaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues V
DOIs
StatePublished - 2007
EventImaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues V - San Jose, CA, United States
Duration: 22 Jan 200724 Jan 2007

Publication series

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume6441
ISSN (Print)1605-7422

Conference

ConferenceImaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues V
Country/TerritoryUnited States
CitySan Jose, CA
Period22/01/0724/01/07

Keywords

  • Aganglionosis
  • Colon
  • Hirschsprung's disease
  • Intelligent algorithms
  • K-means
  • Multi-spectral
  • Parathyroid gland
  • Spectral signature
  • Thyroid gland

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