In vivo-like microsystem for high contents anti-inflammatory drug screening

Microfluidic channels for the in vivo-like inflammatory system was fabricated. After confrim the expression of surface molecules in the microfluidic channel via immunocytochemistry, either activated or inhibited Jurkat T cells were perfused at 3 dyn/cm² of shear stress into the device. The anti-inflammatory drug treated T cells showed the less binding to HUVECs, this indicated that our in vivo-like inflammatory microsystem can detect the binding difference among Jurkat T cells. Therefore, this system is useful for clinical diagnostics as well as high contents drug screening (HCS) on which pharmaceutical industry concentrates recently.