Identification of differentially expressed genes in flower buds of Calanthe discolor and C. sieboldii

Joon Moh Park; Sung Soo Whang; Soonku So; Pyung Ok Lim; Hyo Yeon Lee; Ja Choon Koo

doi:10.1007/s12374-009-9082-2

Identification of differentially expressed genes in flower buds of Calanthe discolor and C. sieboldii

Joon Moh Park
, Sung Soo Whang
, Soonku So
, Pyung Ok Lim
, Hyo Yeon Lee
, Ja Choon Koo

Department of New Biology

Research output: Contribution to journal › Article › peer-review

6 Scopus citations

Abstract

The genus Calanthe includes species of terrestrial orchids that produce attractive flowers with diverse floral traits. Breeding programs have been established to improve the horticultural value of various Calanthe species, but studies to identify the genetic components contributing to the key phenotypic characteristics have not been undertaken. To understand the molecular mechanisms underlying floral development associated with floral morphology, color, and fragrance production, the flower buds of two typical Korean Calanthe species, C. discolor and C. sieboldii, were subjected to gene expression analysis by differential display RT-PCR (DDRT-PCR). A total of 66 non-redundant differentially expressed genes (DEGs) were isolated and sequenced. Of these, 26 and 40 DEGs were found to be highly expressed in C. discolor and C. sieboldii, respectively. Moreover, the expression patterns of a subset of genes presumably implicated in signal transduction, metabolic pathways, and hormonal signaling differed between the two species. The data presented here may improve our understanding of the mechanisms underlying floral development and contribute to advances in orchid biotechnology.

Original language	English
Pages (from-to)	24-31
Number of pages	8
Journal	Journal of Plant Biology
Volume	53
Issue number	1
DOIs	https://doi.org/10.1007/s12374-009-9082-2
State	Published - Feb 2010

Bibliographical note

Funding Information:
Acknowledgements We thank Dr. Hwang Inhwan (POSTECH, Korea) for providing the pBluescript-T vector. This work was supported by research funds from Chonbuk National University in 2007 (NP-2007-180103), the Korea Research Foundation Grant (KRF2007-412-J05501), and a grant from the Jeonbuk Forest Environment Research Institute.

Keywords

DDRT-PCR
Differentially expressed gene (DEG)
Floral gene

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10.1007/s12374-009-9082-2

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title = "Identification of differentially expressed genes in flower buds of Calanthe discolor and C. sieboldii",

abstract = "The genus Calanthe includes species of terrestrial orchids that produce attractive flowers with diverse floral traits. Breeding programs have been established to improve the horticultural value of various Calanthe species, but studies to identify the genetic components contributing to the key phenotypic characteristics have not been undertaken. To understand the molecular mechanisms underlying floral development associated with floral morphology, color, and fragrance production, the flower buds of two typical Korean Calanthe species, C. discolor and C. sieboldii, were subjected to gene expression analysis by differential display RT-PCR (DDRT-PCR). A total of 66 non-redundant differentially expressed genes (DEGs) were isolated and sequenced. Of these, 26 and 40 DEGs were found to be highly expressed in C. discolor and C. sieboldii, respectively. Moreover, the expression patterns of a subset of genes presumably implicated in signal transduction, metabolic pathways, and hormonal signaling differed between the two species. The data presented here may improve our understanding of the mechanisms underlying floral development and contribute to advances in orchid biotechnology.",

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author = "Park, \{Joon Moh\} and Whang, \{Sung Soo\} and Soonku So and Lim, \{Pyung Ok\} and Lee, \{Hyo Yeon\} and Koo, \{Ja Choon\}",

note = "Funding Information: Acknowledgements We thank Dr. Hwang Inhwan (POSTECH, Korea) for providing the pBluescript-T vector. This work was supported by research funds from Chonbuk National University in 2007 (NP-2007-180103), the Korea Research Foundation Grant (KRF2007-412-J05501), and a grant from the Jeonbuk Forest Environment Research Institute.",

year = "2010",

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doi = "10.1007/s12374-009-9082-2",

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AU - Park, Joon Moh

AU - Whang, Sung Soo

AU - So, Soonku

AU - Lim, Pyung Ok

AU - Lee, Hyo Yeon

AU - Koo, Ja Choon

N1 - Funding Information: Acknowledgements We thank Dr. Hwang Inhwan (POSTECH, Korea) for providing the pBluescript-T vector. This work was supported by research funds from Chonbuk National University in 2007 (NP-2007-180103), the Korea Research Foundation Grant (KRF2007-412-J05501), and a grant from the Jeonbuk Forest Environment Research Institute.

PY - 2010/2

Y1 - 2010/2

N2 - The genus Calanthe includes species of terrestrial orchids that produce attractive flowers with diverse floral traits. Breeding programs have been established to improve the horticultural value of various Calanthe species, but studies to identify the genetic components contributing to the key phenotypic characteristics have not been undertaken. To understand the molecular mechanisms underlying floral development associated with floral morphology, color, and fragrance production, the flower buds of two typical Korean Calanthe species, C. discolor and C. sieboldii, were subjected to gene expression analysis by differential display RT-PCR (DDRT-PCR). A total of 66 non-redundant differentially expressed genes (DEGs) were isolated and sequenced. Of these, 26 and 40 DEGs were found to be highly expressed in C. discolor and C. sieboldii, respectively. Moreover, the expression patterns of a subset of genes presumably implicated in signal transduction, metabolic pathways, and hormonal signaling differed between the two species. The data presented here may improve our understanding of the mechanisms underlying floral development and contribute to advances in orchid biotechnology.

AB - The genus Calanthe includes species of terrestrial orchids that produce attractive flowers with diverse floral traits. Breeding programs have been established to improve the horticultural value of various Calanthe species, but studies to identify the genetic components contributing to the key phenotypic characteristics have not been undertaken. To understand the molecular mechanisms underlying floral development associated with floral morphology, color, and fragrance production, the flower buds of two typical Korean Calanthe species, C. discolor and C. sieboldii, were subjected to gene expression analysis by differential display RT-PCR (DDRT-PCR). A total of 66 non-redundant differentially expressed genes (DEGs) were isolated and sequenced. Of these, 26 and 40 DEGs were found to be highly expressed in C. discolor and C. sieboldii, respectively. Moreover, the expression patterns of a subset of genes presumably implicated in signal transduction, metabolic pathways, and hormonal signaling differed between the two species. The data presented here may improve our understanding of the mechanisms underlying floral development and contribute to advances in orchid biotechnology.

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JO - Journal of Plant Biology

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Identification of differentially expressed genes in flower buds of Calanthe discolor and C. sieboldii

Abstract

Bibliographical note

Keywords

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