Abstract
This report describes the fabrication of a novel microfluidics nanobiochip based on a composite comprising of nickel oxide nanoparticles (nNiO) and multiwalled carbon nanotubes (MWCNTs), as well as the chip's use in a biomedical application. This nanocomposite was integrated with polydimethylsiloxane (PDMS) microchannels, which were constructed using the photolithographic technique. A structural and morphological characterization of the fabricated microfluidics chip, which was functionalized with a bienzyme containing cholesterol oxidase (ChOx) and cholesterol esterase (ChEt), was accomplished using X-ray photoelectron spectroscopy (XPS) and scanning electron microscopy. The XPS studies revealed that 9.3% of the carboxyl (COOH) groups present in the nNiO-MWCNT composite are used to form amide bonds with the NH 2 groups of the bienzyme. The response studies on this nanobiochip reveal good reproducibility and selectivity, and a high sensitivity of 2.2â.mA/mM/cm 2. This integrated microfluidics biochip provides a promising low-cost platform for the rapid detection of biomolecules using minute samples.
| Original language | English |
|---|---|
| Article number | 2661 |
| Journal | Scientific Reports |
| Volume | 3 |
| DOIs | |
| State | Published - 2013 |
Bibliographical note
Funding Information:The authors would like to thank the Director of the NPL, New Delhi, India, for the use of their facilities. Md. Azahar Ali and Saurabh Srivastava are thankful to CSIR, India, for the award of Senior Research Fellowships. Financial support was received from the Department of Science and Technology from the government of India (DST/TSG/ME/2008/18), the Indian Council of Medical Research (ICMR/RHN/ADHOC/5/2012-2013), and the Ministry of Education, Science and Technology (WCP program, R32-20026) of Korea. V. V. Agrawal would like to thank the CSIR Empower project for providing funding. The authors also thank Shiju Abraham, of BHU, UP, for the Raman studies.