High-frequency dual mode pulsed wave Doppler imaging for monitoring the functional regeneration of adult zebrafish hearts

Bong Jin Kang, Jinhyoung Park, Jieun Kim, Hyung Ham Kim, Changyang Lee, Jae Youn Hwang, Ching Ling Lien, K. Kirk Shung

Research output: Contribution to journalArticlepeer-review

16 Scopus citations

Abstract

Adult zebrafish is awell-known small animal model for studying heart regeneration. Although the regeneration of scars made by resecting the ventricular apex has been visualized with histological methods, there is no adequate imaging tool for tracking the functional recovery of the damaged heart. For this reason, high-frequency Doppler echocardiography using dual mode pulsed wave Doppler, which provides both tissue Doppler (TD) and Doppler flowin a same cardiac cycle, is developed with a 30 MHzhigh-frequency array ultrasound imaging system. Phantom studies show that the Doppler flow mode of the dual mode is capable of measuring the flow velocity from 0.1 to 15 cm s21 with high accuracy ( p-value = 0.974 > 0.05). In the in vivo study of zebrafish, both TD and Doppler flow signals were simultaneously obtained from the zebrafish heart for the first time, and the synchronized valve motions with the blood flow signals were identified. In the longitudinal study on the zebrafish heart regeneration, the parameters for diagnosing the diastolic dysfunction, for example, E/Em < 10, E/A < 0.14 for wild-type zebrafish, were measured, and the type of diastolic dysfunction caused by the amputation was found to be similar to the restrictive filling. The diastolic function was fully recovered within four weeks post-amputation.

Original languageEnglish
Article number20141154
JournalJournal of the Royal Society Interface
Volume12
Issue number103
DOIs
StatePublished - 6 Feb 2015

Bibliographical note

Publisher Copyright:
© 2014 The Author(s) Published by the Royal Society. All rights reserved.

Keywords

  • Doppler flow
  • Echocardiography
  • Heart regeneration
  • Tissue doppler
  • Zebrafish

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