Abstract
Precise control over interfacial chemistry between nanoparticles and other materials remains a major challenge that limits broad application of nanotechnology in biology. To address this challenge, we used 'steric exclusion' to completely convert commercial quantum dots (QDs) into monovalent imaging probes by wrapping each QD with a functionalized oligonucleotide. We demonstrated the utility of these QDs as modular and nonperturbing imaging probes by tracking individual Notch receptors on live cells.
| Original language | English |
|---|---|
| Pages (from-to) | 1203-1205 |
| Number of pages | 3 |
| Journal | Nature Methods |
| Volume | 10 |
| Issue number | 12 |
| DOIs | |
| State | Published - Dec 2013 |
Bibliographical note
Funding Information:We thank B. van Lengerich, L.D. Hughes and P. Haggie for assistance with single-particle-tracking software. S. Blacklow (Harvard University) provided human Notch1 constructs. R. Vale (University of California San Francisco) provided feedback on the single-molecule imaging. A.P. Alivisatos (University of California Berkeley) provided helpful discussion. J. Taunton and members of Cardiovascular Research Institute Core provided TIRF microscopes. N. Sturman helped with localization microscopy plugin. B. Liang, K. Southard and M. Todhunter generated some preliminary data. J.F. was supported by the University of California San Francisco Center for Synthetic and Systems Biology (P50 GM081879). D.S. was supported by Human Frontier Science Program Cross-disciplinary postdoc research fellowship. K.E.B. was supported by US National Institutes of Health National Research Service Award grant (5F32CA165620). Z.J.G. was supported by Kimmel Family Foundation. Y.J. was partly supported by 1R21EB015088-01 from the US National Institutes of Health NIBIB, and the Bryan Hemming Fellowship.