Expression of human olfactory receptor 10J5 in heart aorta, coronary artery, and endothelial cells and its functional role in angiogenesis

Sung Hee Kim; Yeo Cho Yoon; Ae Sin Lee; Nana Kang; Jaehyung Koo; Mee Ra Rhyu; Jae Ho Park

doi:10.1016/j.bbrc.2015.03.046

Expression of human olfactory receptor 10J5 in heart aorta, coronary artery, and endothelial cells and its functional role in angiogenesis

Sung Hee Kim
, Yeo Cho Yoon
, Ae Sin Lee
, Nana Kang
, Jaehyung Koo
, Mee Ra Rhyu
, Jae Ho Park

Department of New Biology

Research output: Contribution to journal › Article › peer-review

69 Scopus citations

Abstract

ORs are ectopically expressed in non-chemosensory tissues including muscle, kidney, and keratinocytes; however, their physiological roles are largely unknown. We found that human olfactory receptor 10J5 (OR10J5) is expressed in the human aorta, coronary artery, and umbilical vein endothelial cells (HUVEC). Lyral induces Ca²⁺ and phosphorylation of AKT in HUVEC. A knockdown study showed the inhibition of the lyral-induced Ca²⁺ and the phosphorylation AKT and implied that these processes are mediated by OR10J5. In addition, lyral enhanced migration of HUVEC, which were also inhibited by RNAi in a migration assay. In addition, matrigel plug assay showed that lyral enhanced angiogenesis in vivo. Together these data demonstrate the physiological role of OR10J5 in angiogenesis and represent roles of ORs in HUVEC cells.

Original language	English
Pages (from-to)	404-408
Number of pages	5
Journal	Biochemical and Biophysical Research Communications
Volume	460
Issue number	2
DOIs	https://doi.org/10.1016/j.bbrc.2015.03.046
State	Published - 1 May 2015

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© 2015 Elsevier Inc. All rights reserved.

Keywords

AKT
Angiogenesis
Calcium
HUVEC
OR10J5
Olfactory receptor

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10.1016/j.bbrc.2015.03.046

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title = "Expression of human olfactory receptor 10J5 in heart aorta, coronary artery, and endothelial cells and its functional role in angiogenesis",

abstract = "ORs are ectopically expressed in non-chemosensory tissues including muscle, kidney, and keratinocytes; however, their physiological roles are largely unknown. We found that human olfactory receptor 10J5 (OR10J5) is expressed in the human aorta, coronary artery, and umbilical vein endothelial cells (HUVEC). Lyral induces Ca2+ and phosphorylation of AKT in HUVEC. A knockdown study showed the inhibition of the lyral-induced Ca2+ and the phosphorylation AKT and implied that these processes are mediated by OR10J5. In addition, lyral enhanced migration of HUVEC, which were also inhibited by RNAi in a migration assay. In addition, matrigel plug assay showed that lyral enhanced angiogenesis in vivo. Together these data demonstrate the physiological role of OR10J5 in angiogenesis and represent roles of ORs in HUVEC cells.",

keywords = "AKT, Angiogenesis, Calcium, HUVEC, OR10J5, Olfactory receptor",

author = "Kim, \{Sung Hee\} and Yoon, \{Yeo Cho\} and Lee, \{Ae Sin\} and Nana Kang and Jaehyung Koo and Rhyu, \{Mee Ra\} and Park, \{Jae Ho\}",

year = "2015",

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doi = "10.1016/j.bbrc.2015.03.046",

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journal = "Biochemical and Biophysical Research Communications",

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TY - JOUR

T1 - Expression of human olfactory receptor 10J5 in heart aorta, coronary artery, and endothelial cells and its functional role in angiogenesis

AU - Kim, Sung Hee

AU - Yoon, Yeo Cho

AU - Lee, Ae Sin

AU - Kang, Nana

AU - Koo, Jaehyung

AU - Rhyu, Mee Ra

AU - Park, Jae Ho

PY - 2015/5/1

Y1 - 2015/5/1

N2 - ORs are ectopically expressed in non-chemosensory tissues including muscle, kidney, and keratinocytes; however, their physiological roles are largely unknown. We found that human olfactory receptor 10J5 (OR10J5) is expressed in the human aorta, coronary artery, and umbilical vein endothelial cells (HUVEC). Lyral induces Ca2+ and phosphorylation of AKT in HUVEC. A knockdown study showed the inhibition of the lyral-induced Ca2+ and the phosphorylation AKT and implied that these processes are mediated by OR10J5. In addition, lyral enhanced migration of HUVEC, which were also inhibited by RNAi in a migration assay. In addition, matrigel plug assay showed that lyral enhanced angiogenesis in vivo. Together these data demonstrate the physiological role of OR10J5 in angiogenesis and represent roles of ORs in HUVEC cells.

AB - ORs are ectopically expressed in non-chemosensory tissues including muscle, kidney, and keratinocytes; however, their physiological roles are largely unknown. We found that human olfactory receptor 10J5 (OR10J5) is expressed in the human aorta, coronary artery, and umbilical vein endothelial cells (HUVEC). Lyral induces Ca2+ and phosphorylation of AKT in HUVEC. A knockdown study showed the inhibition of the lyral-induced Ca2+ and the phosphorylation AKT and implied that these processes are mediated by OR10J5. In addition, lyral enhanced migration of HUVEC, which were also inhibited by RNAi in a migration assay. In addition, matrigel plug assay showed that lyral enhanced angiogenesis in vivo. Together these data demonstrate the physiological role of OR10J5 in angiogenesis and represent roles of ORs in HUVEC cells.

KW - AKT

KW - Angiogenesis

KW - Calcium

KW - HUVEC

KW - OR10J5

KW - Olfactory receptor

UR - https://www.scopus.com/pages/publications/84937759975

U2 - 10.1016/j.bbrc.2015.03.046

DO - 10.1016/j.bbrc.2015.03.046

M3 - Article

C2 - 25791473

AN - SCOPUS:84937759975

SN - 0006-291X

VL - 460

SP - 404

EP - 408

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

IS - 2

ER -

Expression of human olfactory receptor 10J5 in heart aorta, coronary artery, and endothelial cells and its functional role in angiogenesis

Abstract

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Keywords

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