Effect of plant growth regulator combination and culture period on in vitro regeneration of spinach (Spinacia oleracea L.)

Quyen Van Nguyen; Hyeon Jin Sun; Kyung Hwan Boo; Doseung Lee; Ji Hyun Lee; Pyung Ok Lim; Hyo Yeon Lee; Key Zung Riu; Dong Sun Lee

doi:10.1007/s11816-012-0242-3

Effect of plant growth regulator combination and culture period on in vitro regeneration of spinach (Spinacia oleracea L.)

Quyen Van Nguyen
, Hyeon Jin Sun
, Kyung Hwan Boo
, Doseung Lee
, Ji Hyun Lee
, Pyung Ok Lim
, Hyo Yeon Lee
, Key Zung Riu
, Dong Sun Lee

Department of New Biology

Jeju National University

Research output: Contribution to journal › Article › peer-review

15 Scopus citations

Abstract

The objective of this study was to develop an efficient system for the regeneration of spinach plants (Spinacia oleracea L.) by investigating the factors influencing callus and shoot induction. All plant growth regulator (PGR) combinations tested induced callus with high frequency (73-100 %), and the combination of 5 μM α-naphthaleneacetic acid (NAA), 10 μM 6-benzyladenine (BA) and 0. 1 μM gibberellic acid (GA₃) had the most significant effect on callus growth in term of weight (120. 98 ± 22. 56 mg). A high auxin-containing medium induced competent callus for shoot formation, while high cytokinin-containing media enhanced callus growth and made callus incompetent for shoot regeneration. Longer periods of callus induction in a high auxin-containing medium were required to form competent callus and led to a high regeneration capacity. The PGR combination shift from a high auxin to cytokinin ratio (ACR) to a low ACR resulted in highly efficient regeneration. Among the regeneration systems tested, the combination of 10 μM NAA and 0. 3 μM GA₃ for callus induction for 6 weeks followed by 2 μM NAA and 5 μM BA resulted in the highest plant regeneration frequency (83. 33 ± 6. 43 %) and the highest number of plantlets per explant (7. 93 ± 1. 24). Somatic embryos at cotyledonary stage and plantlets were transferred to PGR-free medium to establish whole plants. Regenerated female plants grew well to maturity in the greenhouse (77. 17 ± 9. 80 %) and produced seeds (175. 21 ± 28. 01 firm seeds per plant).

Original language	English
Pages (from-to)	99-108
Number of pages	10
Journal	Arthropod-Plant Interactions
Volume	7
Issue number	1
DOIs	https://doi.org/10.1007/s11816-012-0242-3
State	Published - Jan 2013

Bibliographical note

Funding Information:
This work was supported by the grants from Priority Research Centers Program (2011-0030748) and Mid-career Researcher Program (2011-0027519) through the National Research Foundation of Korea (NRF) funded by the Ministry of Education, Science and Technology, and the Next-Generation BioGreen 21 Program (SSAC, PJ008122; TAGC, PJ009094), Rural Development Administration, Republic of Korea.

Keywords

Culture period
Plant growth regulator combination
Plant regeneration
Somatic embryogenesis
Spinach

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title = "Effect of plant growth regulator combination and culture period on in vitro regeneration of spinach (Spinacia oleracea L.)",

abstract = "The objective of this study was to develop an efficient system for the regeneration of spinach plants (Spinacia oleracea L.) by investigating the factors influencing callus and shoot induction. All plant growth regulator (PGR) combinations tested induced callus with high frequency (73-100 \%), and the combination of 5 μM α-naphthaleneacetic acid (NAA), 10 μM 6-benzyladenine (BA) and 0. 1 μM gibberellic acid (GA3) had the most significant effect on callus growth in term of weight (120. 98 ± 22. 56 mg). A high auxin-containing medium induced competent callus for shoot formation, while high cytokinin-containing media enhanced callus growth and made callus incompetent for shoot regeneration. Longer periods of callus induction in a high auxin-containing medium were required to form competent callus and led to a high regeneration capacity. The PGR combination shift from a high auxin to cytokinin ratio (ACR) to a low ACR resulted in highly efficient regeneration. Among the regeneration systems tested, the combination of 10 μM NAA and 0. 3 μM GA3 for callus induction for 6 weeks followed by 2 μM NAA and 5 μM BA resulted in the highest plant regeneration frequency (83. 33 ± 6. 43 \%) and the highest number of plantlets per explant (7. 93 ± 1. 24). Somatic embryos at cotyledonary stage and plantlets were transferred to PGR-free medium to establish whole plants. Regenerated female plants grew well to maturity in the greenhouse (77. 17 ± 9. 80 \%) and produced seeds (175. 21 ± 28. 01 firm seeds per plant).",

keywords = "Culture period, Plant growth regulator combination, Plant regeneration, Somatic embryogenesis, Spinach",

author = "Nguyen, \{Quyen Van\} and Sun, \{Hyeon Jin\} and Boo, \{Kyung Hwan\} and Doseung Lee and Lee, \{Ji Hyun\} and Lim, \{Pyung Ok\} and Lee, \{Hyo Yeon\} and Riu, \{Key Zung\} and Lee, \{Dong Sun\}",

note = "Funding Information: This work was supported by the grants from Priority Research Centers Program (2011-0030748) and Mid-career Researcher Program (2011-0027519) through the National Research Foundation of Korea (NRF) funded by the Ministry of Education, Science and Technology, and the Next-Generation BioGreen 21 Program (SSAC, PJ008122; TAGC, PJ009094), Rural Development Administration, Republic of Korea.",

year = "2013",

month = jan,

doi = "10.1007/s11816-012-0242-3",

language = "English",

volume = "7",

pages = "99--108",

journal = "Arthropod-Plant Interactions",

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publisher = "Springer Science and Business Media B.V.",

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T1 - Effect of plant growth regulator combination and culture period on in vitro regeneration of spinach (Spinacia oleracea L.)

AU - Nguyen, Quyen Van

AU - Sun, Hyeon Jin

AU - Boo, Kyung Hwan

AU - Lee, Doseung

AU - Lee, Ji Hyun

AU - Lim, Pyung Ok

AU - Lee, Hyo Yeon

AU - Riu, Key Zung

AU - Lee, Dong Sun

N1 - Funding Information: This work was supported by the grants from Priority Research Centers Program (2011-0030748) and Mid-career Researcher Program (2011-0027519) through the National Research Foundation of Korea (NRF) funded by the Ministry of Education, Science and Technology, and the Next-Generation BioGreen 21 Program (SSAC, PJ008122; TAGC, PJ009094), Rural Development Administration, Republic of Korea.

PY - 2013/1

Y1 - 2013/1

N2 - The objective of this study was to develop an efficient system for the regeneration of spinach plants (Spinacia oleracea L.) by investigating the factors influencing callus and shoot induction. All plant growth regulator (PGR) combinations tested induced callus with high frequency (73-100 %), and the combination of 5 μM α-naphthaleneacetic acid (NAA), 10 μM 6-benzyladenine (BA) and 0. 1 μM gibberellic acid (GA3) had the most significant effect on callus growth in term of weight (120. 98 ± 22. 56 mg). A high auxin-containing medium induced competent callus for shoot formation, while high cytokinin-containing media enhanced callus growth and made callus incompetent for shoot regeneration. Longer periods of callus induction in a high auxin-containing medium were required to form competent callus and led to a high regeneration capacity. The PGR combination shift from a high auxin to cytokinin ratio (ACR) to a low ACR resulted in highly efficient regeneration. Among the regeneration systems tested, the combination of 10 μM NAA and 0. 3 μM GA3 for callus induction for 6 weeks followed by 2 μM NAA and 5 μM BA resulted in the highest plant regeneration frequency (83. 33 ± 6. 43 %) and the highest number of plantlets per explant (7. 93 ± 1. 24). Somatic embryos at cotyledonary stage and plantlets were transferred to PGR-free medium to establish whole plants. Regenerated female plants grew well to maturity in the greenhouse (77. 17 ± 9. 80 %) and produced seeds (175. 21 ± 28. 01 firm seeds per plant).

AB - The objective of this study was to develop an efficient system for the regeneration of spinach plants (Spinacia oleracea L.) by investigating the factors influencing callus and shoot induction. All plant growth regulator (PGR) combinations tested induced callus with high frequency (73-100 %), and the combination of 5 μM α-naphthaleneacetic acid (NAA), 10 μM 6-benzyladenine (BA) and 0. 1 μM gibberellic acid (GA3) had the most significant effect on callus growth in term of weight (120. 98 ± 22. 56 mg). A high auxin-containing medium induced competent callus for shoot formation, while high cytokinin-containing media enhanced callus growth and made callus incompetent for shoot regeneration. Longer periods of callus induction in a high auxin-containing medium were required to form competent callus and led to a high regeneration capacity. The PGR combination shift from a high auxin to cytokinin ratio (ACR) to a low ACR resulted in highly efficient regeneration. Among the regeneration systems tested, the combination of 10 μM NAA and 0. 3 μM GA3 for callus induction for 6 weeks followed by 2 μM NAA and 5 μM BA resulted in the highest plant regeneration frequency (83. 33 ± 6. 43 %) and the highest number of plantlets per explant (7. 93 ± 1. 24). Somatic embryos at cotyledonary stage and plantlets were transferred to PGR-free medium to establish whole plants. Regenerated female plants grew well to maturity in the greenhouse (77. 17 ± 9. 80 %) and produced seeds (175. 21 ± 28. 01 firm seeds per plant).

KW - Culture period

KW - Plant growth regulator combination

KW - Plant regeneration

KW - Somatic embryogenesis

KW - Spinach

UR - https://www.scopus.com/pages/publications/84872320272

U2 - 10.1007/s11816-012-0242-3

DO - 10.1007/s11816-012-0242-3

M3 - Article

AN - SCOPUS:84872320272

SN - 1872-8855

VL - 7

SP - 99

EP - 108

JO - Arthropod-Plant Interactions

JF - Arthropod-Plant Interactions

IS - 1

ER -

Effect of plant growth regulator combination and culture period on in vitro regeneration of spinach (Spinacia oleracea L.)

Abstract

Bibliographical note

Keywords

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