Differential subcellular localization of DNA topoisomerase-1 isoforms and their roles during Caenorhabditis elegans development

Dong Seok Cha; Sarah E. Hollis; Udaya Sree Datla; Sejin Lee; Jinsun Ryu; Hey Rim Jung; Eunsuk Kim; Kyuhyung Kim; Myeongwoo Lee; Chris Li; Myon Hee Lee

doi:10.1016/j.gep.2012.03.002

Differential subcellular localization of DNA topoisomerase-1 isoforms and their roles during Caenorhabditis elegans development

Dong Seok Cha, Sarah E. Hollis, Udaya Sree Datla, Sejin Lee, Jinsun Ryu, Hey Rim Jung, Eunsuk Kim, Kyuhyung Kim, Myeongwoo Lee, Chris Li, Myon Hee Lee

Department of Brain Sciences

Research output: Contribution to journal › Article › peer-review

7 Scopus citations

Abstract

DNA topoisomerase-1 (TOP-1) resolves the topological problems associated with DNA replication, transcription and recombination by introducing temporary single-strand breaks in the DNA. Caenorhabditis elegans TOP-1 has two isoforms, TOP-1α and TOP-1β. TOP-1β is broadly localized to the nuclei of many cells at all developmental stages and concentrated in nucleoli in embryo gut and oogenic cells. However, TOP-1α is specifically localized to centrosomes, neuronal cells, excretory cells and chromosomes of germ cells in embryonic and larval stages. Reporter gene analysis also shows that top-1 transcription is highly activated in several sensory neurons, speculating the possible role of TOP-1α in neuronal development. From RNA interference (RNAi) experiments, we demonstrated that C. elegans TOP-1 is required for chromosomal segregation, germline proliferation and gonadal migration, which are all correlated with the expression and activity of TOP-1. Therefore, our findings may provide an insight into a new role of TOP-1 in development of multicellular organisms.

Original language	English
Pages (from-to)	189-195
Number of pages	7
Journal	Gene Expression Patterns
Volume	12
Issue number	5-6
DOIs	https://doi.org/10.1016/j.gep.2012.03.002
State	Published - May 2012

Bibliographical note

Funding Information:
We thank Dr. Koo (Yonsei University, South Korea) and Dr. Fire (Stanford Univ., USA) for TOP-1 antibodies and the expression vector pPD95.67, respectively. This work was supported by the DGIST Convergence Science Center Program of the Ministry of Education, Science and Technology (11-BD-04) to K.K. and startup funds and East-West research collaboration award from East Carolina University to M.H.L.

Keywords

Caenorhabditis elegans
Chromosomal segregation
Germline development
Neuron
Topoisomerase-1

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10.1016/j.gep.2012.03.002

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@article{9e5598e2303c4c7ea9b01c3b639fa05d,

title = "Differential subcellular localization of DNA topoisomerase-1 isoforms and their roles during Caenorhabditis elegans development",

abstract = "DNA topoisomerase-1 (TOP-1) resolves the topological problems associated with DNA replication, transcription and recombination by introducing temporary single-strand breaks in the DNA. Caenorhabditis elegans TOP-1 has two isoforms, TOP-1α and TOP-1β. TOP-1β is broadly localized to the nuclei of many cells at all developmental stages and concentrated in nucleoli in embryo gut and oogenic cells. However, TOP-1α is specifically localized to centrosomes, neuronal cells, excretory cells and chromosomes of germ cells in embryonic and larval stages. Reporter gene analysis also shows that top-1 transcription is highly activated in several sensory neurons, speculating the possible role of TOP-1α in neuronal development. From RNA interference (RNAi) experiments, we demonstrated that C. elegans TOP-1 is required for chromosomal segregation, germline proliferation and gonadal migration, which are all correlated with the expression and activity of TOP-1. Therefore, our findings may provide an insight into a new role of TOP-1 in development of multicellular organisms.",

keywords = "Caenorhabditis elegans, Chromosomal segregation, Germline development, Neuron, Topoisomerase-1",

author = "Cha, {Dong Seok} and Hollis, {Sarah E.} and Datla, {Udaya Sree} and Sejin Lee and Jinsun Ryu and Jung, {Hey Rim} and Eunsuk Kim and Kyuhyung Kim and Myeongwoo Lee and Chris Li and Lee, {Myon Hee}",

note = "Funding Information: We thank Dr. Koo (Yonsei University, South Korea) and Dr. Fire (Stanford Univ., USA) for TOP-1 antibodies and the expression vector pPD95.67, respectively. This work was supported by the DGIST Convergence Science Center Program of the Ministry of Education, Science and Technology (11-BD-04) to K.K. and startup funds and East-West research collaboration award from East Carolina University to M.H.L. ",

year = "2012",

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doi = "10.1016/j.gep.2012.03.002",

language = "English",

volume = "12",

pages = "189--195",

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AU - Cha, Dong Seok

AU - Hollis, Sarah E.

AU - Datla, Udaya Sree

AU - Lee, Sejin

AU - Ryu, Jinsun

AU - Jung, Hey Rim

AU - Kim, Eunsuk

AU - Kim, Kyuhyung

AU - Lee, Myeongwoo

AU - Li, Chris

AU - Lee, Myon Hee

N1 - Funding Information: We thank Dr. Koo (Yonsei University, South Korea) and Dr. Fire (Stanford Univ., USA) for TOP-1 antibodies and the expression vector pPD95.67, respectively. This work was supported by the DGIST Convergence Science Center Program of the Ministry of Education, Science and Technology (11-BD-04) to K.K. and startup funds and East-West research collaboration award from East Carolina University to M.H.L.

PY - 2012/5

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N2 - DNA topoisomerase-1 (TOP-1) resolves the topological problems associated with DNA replication, transcription and recombination by introducing temporary single-strand breaks in the DNA. Caenorhabditis elegans TOP-1 has two isoforms, TOP-1α and TOP-1β. TOP-1β is broadly localized to the nuclei of many cells at all developmental stages and concentrated in nucleoli in embryo gut and oogenic cells. However, TOP-1α is specifically localized to centrosomes, neuronal cells, excretory cells and chromosomes of germ cells in embryonic and larval stages. Reporter gene analysis also shows that top-1 transcription is highly activated in several sensory neurons, speculating the possible role of TOP-1α in neuronal development. From RNA interference (RNAi) experiments, we demonstrated that C. elegans TOP-1 is required for chromosomal segregation, germline proliferation and gonadal migration, which are all correlated with the expression and activity of TOP-1. Therefore, our findings may provide an insight into a new role of TOP-1 in development of multicellular organisms.

AB - DNA topoisomerase-1 (TOP-1) resolves the topological problems associated with DNA replication, transcription and recombination by introducing temporary single-strand breaks in the DNA. Caenorhabditis elegans TOP-1 has two isoforms, TOP-1α and TOP-1β. TOP-1β is broadly localized to the nuclei of many cells at all developmental stages and concentrated in nucleoli in embryo gut and oogenic cells. However, TOP-1α is specifically localized to centrosomes, neuronal cells, excretory cells and chromosomes of germ cells in embryonic and larval stages. Reporter gene analysis also shows that top-1 transcription is highly activated in several sensory neurons, speculating the possible role of TOP-1α in neuronal development. From RNA interference (RNAi) experiments, we demonstrated that C. elegans TOP-1 is required for chromosomal segregation, germline proliferation and gonadal migration, which are all correlated with the expression and activity of TOP-1. Therefore, our findings may provide an insight into a new role of TOP-1 in development of multicellular organisms.

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ER -

Differential subcellular localization of DNA topoisomerase-1 isoforms and their roles during Caenorhabditis elegans development

Abstract

Bibliographical note

Keywords

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