Abstract
We propose a new in vivo confocal microscope capable of reflection and fluorescence imaging simultaneously with the use of 488 nm, 660 nm, and 830 nm lasers. We measured the lateral resolution of less than 0.8μm, the axial resolution of less than 3.5lμ, the focal shift of 1.25μm, and the imaging depth up to 210μm. Ex vivo and in vivo images are acquired from bovine artery endothelial cell, mouse, and human skin tissues. Two imaging modes can provide potentials that the accuracy of diagnosis can be improved from different point of views.
Original language | English |
---|---|
Pages (from-to) | 325-341 |
Number of pages | 17 |
Journal | International Journal of Optomechatronics |
Volume | 4 |
Issue number | 4 |
DOIs | |
State | Published - Oct 2010 |
Bibliographical note
Funding Information:This study was supported by Technology Development Program for Agriculture and Forestry, Ministry for Agriculture, Forestry and Fisheries, Republic of Korea.
Keywords
- Confocal microscope
- Fluorescence imaging
- In vivo confocal imaging
- Reflection imaging
- Simultaneous reflection and fluorescence imaging