Development of In Vivo confocal microscope combined with reflection and fluorescence imaging modes

Myoung Ki Ahn, Young Duk Kim, Cheol Song, Dae Gab Gweon

Research output: Contribution to journalArticlepeer-review

1 Scopus citations

Abstract

We propose a new in vivo confocal microscope capable of reflection and fluorescence imaging simultaneously with the use of 488 nm, 660 nm, and 830 nm lasers. We measured the lateral resolution of less than 0.8μm, the axial resolution of less than 3.5lμ, the focal shift of 1.25μm, and the imaging depth up to 210μm. Ex vivo and in vivo images are acquired from bovine artery endothelial cell, mouse, and human skin tissues. Two imaging modes can provide potentials that the accuracy of diagnosis can be improved from different point of views.

Original languageEnglish
Pages (from-to)325-341
Number of pages17
JournalInternational Journal of Optomechatronics
Volume4
Issue number4
DOIs
StatePublished - Oct 2010

Bibliographical note

Funding Information:
This study was supported by Technology Development Program for Agriculture and Forestry, Ministry for Agriculture, Forestry and Fisheries, Republic of Korea.

Keywords

  • Confocal microscope
  • Fluorescence imaging
  • In vivo confocal imaging
  • Reflection imaging
  • Simultaneous reflection and fluorescence imaging

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