Abstract
Gram-negative Vibrio species secrete multifunctional autoprocessing repeats-in-toxin (MARTX) toxins associated with bacterial pathogenesis. Here, the cross-reactivity and cross-protectivity of mAbs against V. vulnificus RtxA1/MARTXVv was evaluated. Passive administration of any of these mAbs (21RA, 24RA, 46RA, 47RA and 50RA) provided strong protection against lethal V. cholerae infection. Interestingly, 24RA and 46RA, which map to the cysteine protease domain of V. cholerae MARTXVc, inhibited CPD autocleavage in vitro; this process is involved in V. cholerae pathogenesis. These results generate new insight into the development of broadly protective mAbs and/or vaccines against Vibrio species with MARTX toxins.
Original language | English |
---|---|
Pages (from-to) | 793-800 |
Number of pages | 8 |
Journal | Microbiology and Immunology |
Volume | 60 |
Issue number | 11 |
DOIs | |
State | Published - 1 Nov 2016 |
Bibliographical note
Publisher Copyright:© 2016 The Societies and John Wiley & Sons Australia, Ltd
Keywords
- MARTX toxin
- Vibrio species
- cross-protection
- monoclonal antibody