TY - JOUR
T1 - Clustering assay for studying the interaction of membrane proteins with PDZ domain proteins.
AU - Ko, Jaewon
AU - Kim, Eunjoon
PY - 2006
Y1 - 2006
N2 - Some membrane proteins must be clustered at target sites to efficiently perform their functions. PDZ domain-containing scaffold proteins bind to the tails of target membrane proteins and promote their localization and clustering on the cell surface. This chapter describes the experimental details of the clustering assay, using the interaction between potassium channels and PSD-95, an abundant PDZ domain protein in neuronal synapses, as a model.
AB - Some membrane proteins must be clustered at target sites to efficiently perform their functions. PDZ domain-containing scaffold proteins bind to the tails of target membrane proteins and promote their localization and clustering on the cell surface. This chapter describes the experimental details of the clustering assay, using the interaction between potassium channels and PSD-95, an abundant PDZ domain protein in neuronal synapses, as a model.
UR - http://www.scopus.com/inward/record.url?scp=33747401285&partnerID=8YFLogxK
U2 - 10.1385/1-59745-048-0:245
DO - 10.1385/1-59745-048-0:245
M3 - Article
C2 - 16878697
AN - SCOPUS:33747401285
SN - 1064-3745
VL - 332
SP - 245
EP - 253
JO - Methods in molecular biology (Clifton, N.J.)
JF - Methods in molecular biology (Clifton, N.J.)
ER -