Abstract
Endogenous bone marrow-derived mesenchymal stem cells (BM-MSCs) are mobilized into peripheral blood and injured tissues by various growth factors and cytokines that are expressed in the injured tissues, such as substance P (SP), stromal cell derived factor-1 (SDF-1), and transforming growth factor-beta (TGF-β). Extracellular bioactive lipid metabolites such as ceramide-1-phosphate and sphingosine-1-phosphate also modulate BM-MSC migration as SP, SDF-1, and TGF-β. However, the roles of intrinsic lipid kinases of BM-MSCs in the stem cell migration are unclear. Here, we demonstrated that ceramide kinase mediates the chemotactic migration of BM-MSCs in response to SP, SDF-1, or TGF-β. Furthermore, a specific inhibitor of ceramide kinase inhibited TGF-β-induced migration of BM-MSCs and N-cadherin that is necessary for BM-MSCs migration in response to TGF-β. Therefore, these results suggest that the intracellular ceramide kinase is required for the BM-MSCs migration and the roles of the intrinsic ceramide kinase in the migration are associated with N-cadherin regulation.
| Original language | English |
|---|---|
| Pages (from-to) | 361-367 |
| Number of pages | 7 |
| Journal | Biochemical and Biophysical Research Communications |
| Volume | 508 |
| Issue number | 2 |
| DOIs | |
| State | Published - 8 Jan 2019 |
Bibliographical note
Publisher Copyright:© 2018
Keywords
- Ceramide kinase
- Mesenchymal stem cells
- Migration
- N-cadherin
- TGF-β