Cell reprogramming into the pluripotent state using graphene based substrates

Junsang Yoo; Jongmin Kim; Soonbong Baek; Youngsin Park; Hyunsik Im; Jongpil Kim

doi:10.1016/j.biomaterials.2014.05.096

Cell reprogramming into the pluripotent state using graphene based substrates

Junsang Yoo, Jongmin Kim, Soonbong Baek, Youngsin Park, Hyunsik Im, Jongpil Kim

Division of Energy Techonology

Research output: Contribution to journal › Article › peer-review

57 Scopus citations

Abstract

Graphene has been attracting considerable interest in the field of biomedical engineering because graphene and its derivatives are considered to be ideal platforms for supporting cell growth and differentiation. Here we report that graphene promotes the reprogramming of mouse somatic fibroblasts into induced pluripotent stem cells (iPSCs). We constructed a layer of graphene film on a glass substrate and characterized it as a monolayer using Raman spectroscopy. We found that the graphene substrate significantly improved cellular reprogramming efficiency by inducing mesenchymal-to-epithelial-transition (MET) which is known to affect H3K4me3 levels. Thus, our results reveal that a graphene substrate directly regulates dynamic epigenetic changes associated with reprogramming, providing an efficient tool for epigenetic pluripotent reprogramming.

Original language	English
Pages (from-to)	8321-8329
Number of pages	9
Journal	Biomaterials
Volume	35
Issue number	29
DOIs	https://doi.org/10.1016/j.biomaterials.2014.05.096
State	Published - Sep 2014

Bibliographical note

Funding Information:
This work was supported by the National Research Foundation of Korea funded by the Ministry of Education, Science, and Technology (NRF- 2013R1A1A1058835 , NRF- 2013M3A9B4076485 , NRF- 2013M3A9B4044387 ), Korea Health Technology R&D Project, Ministry of Health & Welfare ( HI13C0540 ) and the Next-Generation BioGreen 21 Program, Rural Development Administration ( PJ009073 ).

Keywords

Graphene
Induced pluripotent stem cells
Mesenchymal-to-epithelial transition
Reprogramming

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10.1016/j.biomaterials.2014.05.096

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title = "Cell reprogramming into the pluripotent state using graphene based substrates",

abstract = "Graphene has been attracting considerable interest in the field of biomedical engineering because graphene and its derivatives are considered to be ideal platforms for supporting cell growth and differentiation. Here we report that graphene promotes the reprogramming of mouse somatic fibroblasts into induced pluripotent stem cells (iPSCs). We constructed a layer of graphene film on a glass substrate and characterized it as a monolayer using Raman spectroscopy. We found that the graphene substrate significantly improved cellular reprogramming efficiency by inducing mesenchymal-to-epithelial-transition (MET) which is known to affect H3K4me3 levels. Thus, our results reveal that a graphene substrate directly regulates dynamic epigenetic changes associated with reprogramming, providing an efficient tool for epigenetic pluripotent reprogramming.",

keywords = "Graphene, Induced pluripotent stem cells, Mesenchymal-to-epithelial transition, Reprogramming",

author = "Junsang Yoo and Jongmin Kim and Soonbong Baek and Youngsin Park and Hyunsik Im and Jongpil Kim",

note = "Funding Information: This work was supported by the National Research Foundation of Korea funded by the Ministry of Education, Science, and Technology (NRF- 2013R1A1A1058835 , NRF- 2013M3A9B4076485 , NRF- 2013M3A9B4044387 ), Korea Health Technology R&D Project, Ministry of Health & Welfare ( HI13C0540 ) and the Next-Generation BioGreen 21 Program, Rural Development Administration ( PJ009073 ). ",

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AU - Yoo, Junsang

AU - Kim, Jongmin

AU - Baek, Soonbong

AU - Park, Youngsin

AU - Im, Hyunsik

AU - Kim, Jongpil

N1 - Funding Information: This work was supported by the National Research Foundation of Korea funded by the Ministry of Education, Science, and Technology (NRF- 2013R1A1A1058835 , NRF- 2013M3A9B4076485 , NRF- 2013M3A9B4044387 ), Korea Health Technology R&D Project, Ministry of Health & Welfare ( HI13C0540 ) and the Next-Generation BioGreen 21 Program, Rural Development Administration ( PJ009073 ).

PY - 2014/9

Y1 - 2014/9

N2 - Graphene has been attracting considerable interest in the field of biomedical engineering because graphene and its derivatives are considered to be ideal platforms for supporting cell growth and differentiation. Here we report that graphene promotes the reprogramming of mouse somatic fibroblasts into induced pluripotent stem cells (iPSCs). We constructed a layer of graphene film on a glass substrate and characterized it as a monolayer using Raman spectroscopy. We found that the graphene substrate significantly improved cellular reprogramming efficiency by inducing mesenchymal-to-epithelial-transition (MET) which is known to affect H3K4me3 levels. Thus, our results reveal that a graphene substrate directly regulates dynamic epigenetic changes associated with reprogramming, providing an efficient tool for epigenetic pluripotent reprogramming.

AB - Graphene has been attracting considerable interest in the field of biomedical engineering because graphene and its derivatives are considered to be ideal platforms for supporting cell growth and differentiation. Here we report that graphene promotes the reprogramming of mouse somatic fibroblasts into induced pluripotent stem cells (iPSCs). We constructed a layer of graphene film on a glass substrate and characterized it as a monolayer using Raman spectroscopy. We found that the graphene substrate significantly improved cellular reprogramming efficiency by inducing mesenchymal-to-epithelial-transition (MET) which is known to affect H3K4me3 levels. Thus, our results reveal that a graphene substrate directly regulates dynamic epigenetic changes associated with reprogramming, providing an efficient tool for epigenetic pluripotent reprogramming.

KW - Graphene

KW - Induced pluripotent stem cells

KW - Mesenchymal-to-epithelial transition

KW - Reprogramming

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SP - 8321

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JO - Biomaterials

JF - Biomaterials

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ER -

Cell reprogramming into the pluripotent state using graphene based substrates

Abstract

Bibliographical note

Keywords

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