Cell block preparation from pleural fluid by an ultrasound-assisted filtration

Tingyu Li; Yaoping Liu; Lianjun Lin; Eunhee Kim; Xinmin Liu; Haichao Li; Hongsoo Choi; Wei Wang

Cell block preparation from pleural fluid by an ultrasound-assisted filtration

Tingyu Li, Yaoping Liu, Lianjun Lin, Eunhee Kim, Xinmin Liu, Haichao Li, Hongsoo Choi, Wei Wang

Department of Robotics and Mechatronics Engineering

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

1 Scopus citations

Abstract

This paper reports a novel ultrasound-assisted filtration technique for cell block (CB) preparation from pleural fluid. CB (> 5×10⁶ cells) from a large-volume (>10 mL) specimen was obtained on the Parylene-C filtration membrane (1.29×10⁶ micropores) with a high recovery rate (>90%), a high cell viability (>96%) and an effective depletion of red blood cells (RBCs). The prepared CB of A549s (cultured lung cancer cells) from PBS and target cells from clinical pleural fluid can be well embedded with paraffin. Successful hematoxylin-eosin (HE), immunocytochemistry (ICC) and immunofluorescence (IF) staining of the CBs sections were demonstrated, showing the promise of application in clinical practices.

Original language	English
Title of host publication	22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018
Publisher	Chemical and Biological Microsystems Society
Pages	1750-1752
Number of pages	3
ISBN (Electronic)	9781510897571
State	Published - 2018
Event	22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018 - Kaohsiung, Taiwan, Province of China Duration: 11 Nov 2018 → 15 Nov 2018

Publication series

Name	22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018
Volume	3

Conference

Conference	22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018
Country/Territory	Taiwan, Province of China
City	Kaohsiung
Period	11/11/18 → 15/11/18

Bibliographical note

Publisher Copyright:
Copyright © (2018) by Chemical and Biological Microsystems Society. All rights reserved.

Keywords

Cell block preparation
Filtration
Pleural fluid

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Cite this

Li, T., Liu, Y., Lin, L., Kim, E., Liu, X., Li, H., Choi, H., & Wang, W. (2018). Cell block preparation from pleural fluid by an ultrasound-assisted filtration. In 22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018 (pp. 1750-1752). (22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018; Vol. 3). Chemical and Biological Microsystems Society.

Li, Tingyu ; Liu, Yaoping ; Lin, Lianjun et al. / Cell block preparation from pleural fluid by an ultrasound-assisted filtration. 22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018. Chemical and Biological Microsystems Society, 2018. pp. 1750-1752 (22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018).

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title = "Cell block preparation from pleural fluid by an ultrasound-assisted filtration",

abstract = "This paper reports a novel ultrasound-assisted filtration technique for cell block (CB) preparation from pleural fluid. CB (> 5×106 cells) from a large-volume (>10 mL) specimen was obtained on the Parylene-C filtration membrane (1.29×106 micropores) with a high recovery rate (>90%), a high cell viability (>96%) and an effective depletion of red blood cells (RBCs). The prepared CB of A549s (cultured lung cancer cells) from PBS and target cells from clinical pleural fluid can be well embedded with paraffin. Successful hematoxylin-eosin (HE), immunocytochemistry (ICC) and immunofluorescence (IF) staining of the CBs sections were demonstrated, showing the promise of application in clinical practices.",

keywords = "Cell block preparation, Filtration, Pleural fluid",

author = "Tingyu Li and Yaoping Liu and Lianjun Lin and Eunhee Kim and Xinmin Liu and Haichao Li and Hongsoo Choi and Wei Wang",

note = "Publisher Copyright: Copyright {\textcopyright} (2018) by Chemical and Biological Microsystems Society. All rights reserved.; 22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018 ; Conference date: 11-11-2018 Through 15-11-2018",

year = "2018",

language = "English",

series = "22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018",

publisher = "Chemical and Biological Microsystems Society",

pages = "1750--1752",

booktitle = "22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018",

}

Li, T, Liu, Y, Lin, L, Kim, E, Liu, X, Li, H, Choi, H & Wang, W 2018, Cell block preparation from pleural fluid by an ultrasound-assisted filtration. in 22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018. 22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018, vol. 3, Chemical and Biological Microsystems Society, pp. 1750-1752, 22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018, Kaohsiung, Taiwan, Province of China, 11/11/18.

Cell block preparation from pleural fluid by an ultrasound-assisted filtration. / Li, Tingyu; Liu, Yaoping; Lin, Lianjun et al.
22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018. Chemical and Biological Microsystems Society, 2018. p. 1750-1752 (22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018; Vol. 3).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

TY - GEN

T1 - Cell block preparation from pleural fluid by an ultrasound-assisted filtration

AU - Li, Tingyu

AU - Liu, Yaoping

AU - Lin, Lianjun

AU - Kim, Eunhee

AU - Liu, Xinmin

AU - Li, Haichao

AU - Choi, Hongsoo

AU - Wang, Wei

PY - 2018

Y1 - 2018

N2 - This paper reports a novel ultrasound-assisted filtration technique for cell block (CB) preparation from pleural fluid. CB (> 5×106 cells) from a large-volume (>10 mL) specimen was obtained on the Parylene-C filtration membrane (1.29×106 micropores) with a high recovery rate (>90%), a high cell viability (>96%) and an effective depletion of red blood cells (RBCs). The prepared CB of A549s (cultured lung cancer cells) from PBS and target cells from clinical pleural fluid can be well embedded with paraffin. Successful hematoxylin-eosin (HE), immunocytochemistry (ICC) and immunofluorescence (IF) staining of the CBs sections were demonstrated, showing the promise of application in clinical practices.

AB - This paper reports a novel ultrasound-assisted filtration technique for cell block (CB) preparation from pleural fluid. CB (> 5×106 cells) from a large-volume (>10 mL) specimen was obtained on the Parylene-C filtration membrane (1.29×106 micropores) with a high recovery rate (>90%), a high cell viability (>96%) and an effective depletion of red blood cells (RBCs). The prepared CB of A549s (cultured lung cancer cells) from PBS and target cells from clinical pleural fluid can be well embedded with paraffin. Successful hematoxylin-eosin (HE), immunocytochemistry (ICC) and immunofluorescence (IF) staining of the CBs sections were demonstrated, showing the promise of application in clinical practices.

KW - Cell block preparation

KW - Filtration

KW - Pleural fluid

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M3 - Conference contribution

AN - SCOPUS:85079810717

T3 - 22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018

SP - 1750

EP - 1752

BT - 22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018

PB - Chemical and Biological Microsystems Society

T2 - 22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018

Y2 - 11 November 2018 through 15 November 2018

ER -

Li T, Liu Y, Lin L, Kim E, Liu X, Li H et al. Cell block preparation from pleural fluid by an ultrasound-assisted filtration. In 22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018. Chemical and Biological Microsystems Society. 2018. p. 1750-1752. (22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018).

Cell block preparation from pleural fluid by an ultrasound-assisted filtration

Abstract

Publication series

Conference

Bibliographical note

Keywords

UN SDGs

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Cite this