Abstract
Antioxidants either scavenge superoxide and free radicals or stimulate the detoxification mechanisms within cells, resulting in increased detoxification of free radicals formation. Hyperin, isolated from the stem of Uncaria rhynchophylla, prevented oxygen radical formation and inhibited lipid oxidation. The effective concentrations were 31.3 μM for a radical scavenging assay and 2.2 μM for a microsome assay. cDNA microarray analysis to determine which genes were modulated by hyperin found that 50 genes were upregulated and 37 genes were downregulated in SNU-668 human gastric cancer cells. Among these genes, thirteen genes that were significantly affected by hyperin were verified by RT-PCR for their effect of genetic reprogramming.
| Original language | English |
|---|---|
| Pages (from-to) | 664-668 |
| Number of pages | 5 |
| Journal | Journal of Microbiology and Biotechnology |
| Volume | 12 |
| Issue number | 4 |
| State | Published - Aug 2002 |
Keywords
- Antioxidant
- Human gastric cancer cell
- Hyperin
- Microarray
- RT-PCR
