Brief guide to senescence assays using cultured mammalian cells

Eunseok Kang; Chanhee Kang; Young Sam Lee; Seung Jae V. Lee

doi:10.1016/j.mocell.2024.100102

Brief guide to senescence assays using cultured mammalian cells

Eunseok Kang
, Chanhee Kang
, Young Sam Lee
, Seung Jae V. Lee

Department of New Biology

Research output: Contribution to journal › Letter › peer-review

17 Scopus citations

Abstract

Cellular senescence is a crucial biological process associated with organismal aging and many chronic diseases. Here, we present a brief guide to mammalian senescence assays, including the measurement of cell cycle arrest, change in cellular morphology, senescence-associated β-galactosidase (SA-β-gal) staining, and the expression of senescence-associated secretory phenotype (SASP). This work will be useful for biologists with minimum expertise in cellular senescence assays.

Original language	English
Article number	100102
Journal	Molecules and Cells
Volume	47
Issue number	9
DOIs	https://doi.org/10.1016/j.mocell.2024.100102
State	Published - Sep 2024

Bibliographical note

Publisher Copyright:
© 2024 The Author(s)

Keywords

Aging
Cell cycle arrest
Senescence
Senescence-associated secretory phenotype
Senescence-associated β-galactosidase

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10.1016/j.mocell.2024.100102

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title = "Brief guide to senescence assays using cultured mammalian cells",

abstract = "Cellular senescence is a crucial biological process associated with organismal aging and many chronic diseases. Here, we present a brief guide to mammalian senescence assays, including the measurement of cell cycle arrest, change in cellular morphology, senescence-associated β-galactosidase (SA-β-gal) staining, and the expression of senescence-associated secretory phenotype (SASP). This work will be useful for biologists with minimum expertise in cellular senescence assays.",

keywords = "Aging, Cell cycle arrest, Senescence, Senescence-associated secretory phenotype, Senescence-associated β-galactosidase",

author = "Eunseok Kang and Chanhee Kang and Lee, \{Young Sam\} and Lee, \{Seung Jae V.\}",

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doi = "10.1016/j.mocell.2024.100102",

language = "English",

volume = "47",

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T1 - Brief guide to senescence assays using cultured mammalian cells

AU - Kang, Eunseok

AU - Kang, Chanhee

AU - Lee, Young Sam

AU - Lee, Seung Jae V.

PY - 2024/9

Y1 - 2024/9

N2 - Cellular senescence is a crucial biological process associated with organismal aging and many chronic diseases. Here, we present a brief guide to mammalian senescence assays, including the measurement of cell cycle arrest, change in cellular morphology, senescence-associated β-galactosidase (SA-β-gal) staining, and the expression of senescence-associated secretory phenotype (SASP). This work will be useful for biologists with minimum expertise in cellular senescence assays.

AB - Cellular senescence is a crucial biological process associated with organismal aging and many chronic diseases. Here, we present a brief guide to mammalian senescence assays, including the measurement of cell cycle arrest, change in cellular morphology, senescence-associated β-galactosidase (SA-β-gal) staining, and the expression of senescence-associated secretory phenotype (SASP). This work will be useful for biologists with minimum expertise in cellular senescence assays.

KW - Aging

KW - Cell cycle arrest

KW - Senescence

KW - Senescence-associated secretory phenotype

KW - Senescence-associated β-galactosidase

UR - https://www.scopus.com/pages/publications/85201194565

U2 - 10.1016/j.mocell.2024.100102

DO - 10.1016/j.mocell.2024.100102

M3 - Letter

C2 - 39053732

AN - SCOPUS:85201194565

SN - 1016-8478

VL - 47

JO - Molecules and Cells

JF - Molecules and Cells

IS - 9

M1 - 100102

ER -

Brief guide to senescence assays using cultured mammalian cells

Abstract

Bibliographical note

Keywords

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