Bex1 knock out mice show altered skeletal muscle regeneration

Jae Hyung Koo; Mark A. Smiley; Richard M. Lovering; Frank L. Margolis

doi:10.1016/j.bbrc.2007.08.186

Bex1 knock out mice show altered skeletal muscle regeneration

Jae Hyung Koo, Mark A. Smiley, Richard M. Lovering, Frank L. Margolis

Department of New Biology

University of Maryland, Baltimore

Research output: Contribution to journal › Article › peer-review

30 Scopus citations

Abstract

Bex1 and Calmodulin (CaM) are upregulated during skeletal muscle regeneration. We confirm this finding and demonstrate the novel finding that they interact in a calcium-dependent manner. To study the role of Bex1 and its interaction with CaM in skeletal muscle regeneration, we generated Bex1 knock out (Bex1-KO) mice. These mice appeared to develop normally and are fertile, but displayed a functional deficit in exercise performance compared to wild type (WT) mice. After intramuscular injection of cardiotoxin, which causes extensive and reproducible myotrauma followed by recovery, regenerating muscles of Bex1-KO mice exhibited elevated and prolonged cell proliferation, as well as delayed cell differentiation, compared to WT mice. Thus, our results provide the first evidence that Bex1-KO mice show altered muscle regeneration, and allow us to propose that the interaction of Bex1 with Ca²⁺/CaM may be involved in skeletal muscle regeneration.

Original language	English
Pages (from-to)	405-410
Number of pages	6
Journal	Biochemical and Biophysical Research Communications
Volume	363
Issue number	2
DOIs	https://doi.org/10.1016/j.bbrc.2007.08.186
State	Published - 16 Nov 2007

Bibliographical note

Funding Information:
The authors thank Dr. Robert J. Bloch, and Joyce W. Margolis for valuable discussions, Valerie Stewart of the UMB Transgenic/Knockout core for ES cell characterization and blastocyst injection and Faith Scipio for technical assistance. This work was supported by NIH Grants R01-03112 (to F.L.M.), K01AR053235 (to R.M.L.), and MDA Grant 4278 (to R.M.L.).

Keywords

Bex1
Bex1-KO mice
CaM-binding protein
Calcium
Calmodulin
Muscle regeneration
Myogenesis
Omnibank

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10.1016/j.bbrc.2007.08.186

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title = "Bex1 knock out mice show altered skeletal muscle regeneration",

abstract = "Bex1 and Calmodulin (CaM) are upregulated during skeletal muscle regeneration. We confirm this finding and demonstrate the novel finding that they interact in a calcium-dependent manner. To study the role of Bex1 and its interaction with CaM in skeletal muscle regeneration, we generated Bex1 knock out (Bex1-KO) mice. These mice appeared to develop normally and are fertile, but displayed a functional deficit in exercise performance compared to wild type (WT) mice. After intramuscular injection of cardiotoxin, which causes extensive and reproducible myotrauma followed by recovery, regenerating muscles of Bex1-KO mice exhibited elevated and prolonged cell proliferation, as well as delayed cell differentiation, compared to WT mice. Thus, our results provide the first evidence that Bex1-KO mice show altered muscle regeneration, and allow us to propose that the interaction of Bex1 with Ca2+/CaM may be involved in skeletal muscle regeneration.",

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note = "Funding Information: The authors thank Dr. Robert J. Bloch, and Joyce W. Margolis for valuable discussions, Valerie Stewart of the UMB Transgenic/Knockout core for ES cell characterization and blastocyst injection and Faith Scipio for technical assistance. This work was supported by NIH Grants R01-03112 (to F.L.M.), K01AR053235 (to R.M.L.), and MDA Grant 4278 (to R.M.L.). ",

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AU - Smiley, Mark A.

AU - Lovering, Richard M.

AU - Margolis, Frank L.

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PY - 2007/11/16

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N2 - Bex1 and Calmodulin (CaM) are upregulated during skeletal muscle regeneration. We confirm this finding and demonstrate the novel finding that they interact in a calcium-dependent manner. To study the role of Bex1 and its interaction with CaM in skeletal muscle regeneration, we generated Bex1 knock out (Bex1-KO) mice. These mice appeared to develop normally and are fertile, but displayed a functional deficit in exercise performance compared to wild type (WT) mice. After intramuscular injection of cardiotoxin, which causes extensive and reproducible myotrauma followed by recovery, regenerating muscles of Bex1-KO mice exhibited elevated and prolonged cell proliferation, as well as delayed cell differentiation, compared to WT mice. Thus, our results provide the first evidence that Bex1-KO mice show altered muscle regeneration, and allow us to propose that the interaction of Bex1 with Ca2+/CaM may be involved in skeletal muscle regeneration.

AB - Bex1 and Calmodulin (CaM) are upregulated during skeletal muscle regeneration. We confirm this finding and demonstrate the novel finding that they interact in a calcium-dependent manner. To study the role of Bex1 and its interaction with CaM in skeletal muscle regeneration, we generated Bex1 knock out (Bex1-KO) mice. These mice appeared to develop normally and are fertile, but displayed a functional deficit in exercise performance compared to wild type (WT) mice. After intramuscular injection of cardiotoxin, which causes extensive and reproducible myotrauma followed by recovery, regenerating muscles of Bex1-KO mice exhibited elevated and prolonged cell proliferation, as well as delayed cell differentiation, compared to WT mice. Thus, our results provide the first evidence that Bex1-KO mice show altered muscle regeneration, and allow us to propose that the interaction of Bex1 with Ca2+/CaM may be involved in skeletal muscle regeneration.

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Bex1 knock out mice show altered skeletal muscle regeneration

Abstract

Bibliographical note

Keywords

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