Antibody immobilized cysteamine functionalized-gold nanoparticles for aflatoxin detection

Aditya Sharma, Zimple Matharu, G. Sumana, Pratima R. Solanki, C. G. Kim, B. D. Malhotra

Research output: Contribution to journalArticlepeer-review

141 Scopus citations

Abstract

Aflatoxin B1 antibody (aAFB1) covalently attached to cysteamine functionalized-gold nanoparticles (C-AuNP) has been immobilized onto 4-mercaptobenzoic acid (MBA) based self assembled monolayer (SAM) on gold electrode (MBA/Au), for the fabrication of BSA/aAFB1-C-AuNP/MBA/Au immunoelectrode. This immunoelectrode has been characterized by Fourier Transform Infrared Spectroscopy (FT-IR), Scanning Electron Microscopy (SEM) and electrochemical characterization techniques. The electrochemical response studies reveal that the BSA/aAFB1-C-AuNP/MBA/Au immunoelectrode can be used to detect AFB1 in the range of 10-100 ng dL- 1 and has sensitivity as 0.45 μA ng- 1 dL, limit of detection as 17.90 ng dL- 1 and a response time of 60 s.

Original languageEnglish
Pages (from-to)1213-1218
Number of pages6
JournalThin Solid Films
Volume519
Issue number3
DOIs
StatePublished - 30 Nov 2010

Bibliographical note

Funding Information:
We thank Prof. R. C. Budhani, Director, National Physical Laboratory, India, for providing the facilities. The authors thank Dr. K. N. Sood, NPL, for SEM measurements. Aditya Sharma is thankful to CSIR, India, for the award of a Junior Research Fellowship. The financial support received from Department of Biotechnology, India under the project GAP-080932 , Department of Science and Technology, India ( GAP-081132 ), in-House projects and the Ministry of Education, Science and Technology, Korea ( R32-20026 ) are sincerely acknowledged.

Keywords

  • Aflatoxin
  • Cysteamine
  • Gold nanoparticles
  • Immunosensor
  • Self assembled monolayer

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