Analytes kinetics in lateral flow membrane analyzed by cTnI monitoring using magnetic method

Sunjong Oh; S. Anandakumar; Chungwan Lee; Kun Woo Kim; Byunghwa Lim; Cheolgi Kim

doi:10.1016/j.snb.2011.08.058

Analytes kinetics in lateral flow membrane analyzed by cTnI monitoring using magnetic method

Sunjong Oh, S. Anandakumar, Chungwan Lee, Kun Woo Kim, Byunghwa Lim, Cheolgi Kim

Department of Physics and Chemistry

Research output: Contribution to journal › Article › peer-review

16 Scopus citations

Abstract

Cardiac Troponin I (cTnI) functionalized immunomagnetic beads in lateral flow membrane was measured to analyze the analyte kinetics of mass-transport and antibody-antigen binding reaction. A hybrid AMR/PHR (Anisotropic magnetoresistance/Planar Hall resistance) ring sensor exhibits self-balanced bridge characteristics for each branch with an additive magnetic field effect and its field sensitivity was 135 μV/Oe for the ± 50 Oe range. The real time monitoring of the sensor voltage for the cTnI concentrations in the range of 0.44-2.2 nM exhibits a two-step increment with time. A rapid increment in early time regime up to 50 s is related with the kinetics of antibody-antigen binding reactions, and then a gradual increment is governed by the kinetics of analyte transportation. The sensor voltage variation in early time regime was equal to 70% of the total variation at 400 s.

Original language	English
Pages (from-to)	747-752
Number of pages	6
Journal	Sensors and Actuators, B: Chemical
Volume	160
Issue number	1
DOIs	https://doi.org/10.1016/j.snb.2011.08.058
State	Published - 15 Dec 2011

Bibliographical note

Funding Information:
This research was supported by the WCU (World Class University) program through the National Research Foundation of Korea funded by the Ministry of Education, Science and Technology ( R32-20026 ).

Keywords

Antibody
Antigen
Cardiac Troponin I
Hybrid AMR/PHR ring sensors
Magnetic beads
NC membrane

Access to Document

10.1016/j.snb.2011.08.058

Cite this

@article{64cd60471ce248309bd22a361580d638,

title = "Analytes kinetics in lateral flow membrane analyzed by cTnI monitoring using magnetic method",

abstract = "Cardiac Troponin I (cTnI) functionalized immunomagnetic beads in lateral flow membrane was measured to analyze the analyte kinetics of mass-transport and antibody-antigen binding reaction. A hybrid AMR/PHR (Anisotropic magnetoresistance/Planar Hall resistance) ring sensor exhibits self-balanced bridge characteristics for each branch with an additive magnetic field effect and its field sensitivity was 135 μV/Oe for the ± 50 Oe range. The real time monitoring of the sensor voltage for the cTnI concentrations in the range of 0.44-2.2 nM exhibits a two-step increment with time. A rapid increment in early time regime up to 50 s is related with the kinetics of antibody-antigen binding reactions, and then a gradual increment is governed by the kinetics of analyte transportation. The sensor voltage variation in early time regime was equal to 70% of the total variation at 400 s.",

keywords = "Antibody, Antigen, Cardiac Troponin I, Hybrid AMR/PHR ring sensors, Magnetic beads, NC membrane",

author = "Sunjong Oh and S. Anandakumar and Chungwan Lee and Kim, {Kun Woo} and Byunghwa Lim and Cheolgi Kim",

note = "Funding Information: This research was supported by the WCU (World Class University) program through the National Research Foundation of Korea funded by the Ministry of Education, Science and Technology ( R32-20026 ). ",

year = "2011",

month = dec,

day = "15",

doi = "10.1016/j.snb.2011.08.058",

language = "English",

volume = "160",

pages = "747--752",

journal = "Sensors and Actuators, B: Chemical",

issn = "0925-4005",

publisher = "Elsevier B.V.",

number = "1",

}

TY - JOUR

T1 - Analytes kinetics in lateral flow membrane analyzed by cTnI monitoring using magnetic method

AU - Oh, Sunjong

AU - Anandakumar, S.

AU - Lee, Chungwan

AU - Kim, Kun Woo

AU - Lim, Byunghwa

AU - Kim, Cheolgi

N1 - Funding Information: This research was supported by the WCU (World Class University) program through the National Research Foundation of Korea funded by the Ministry of Education, Science and Technology ( R32-20026 ).

PY - 2011/12/15

Y1 - 2011/12/15

N2 - Cardiac Troponin I (cTnI) functionalized immunomagnetic beads in lateral flow membrane was measured to analyze the analyte kinetics of mass-transport and antibody-antigen binding reaction. A hybrid AMR/PHR (Anisotropic magnetoresistance/Planar Hall resistance) ring sensor exhibits self-balanced bridge characteristics for each branch with an additive magnetic field effect and its field sensitivity was 135 μV/Oe for the ± 50 Oe range. The real time monitoring of the sensor voltage for the cTnI concentrations in the range of 0.44-2.2 nM exhibits a two-step increment with time. A rapid increment in early time regime up to 50 s is related with the kinetics of antibody-antigen binding reactions, and then a gradual increment is governed by the kinetics of analyte transportation. The sensor voltage variation in early time regime was equal to 70% of the total variation at 400 s.

AB - Cardiac Troponin I (cTnI) functionalized immunomagnetic beads in lateral flow membrane was measured to analyze the analyte kinetics of mass-transport and antibody-antigen binding reaction. A hybrid AMR/PHR (Anisotropic magnetoresistance/Planar Hall resistance) ring sensor exhibits self-balanced bridge characteristics for each branch with an additive magnetic field effect and its field sensitivity was 135 μV/Oe for the ± 50 Oe range. The real time monitoring of the sensor voltage for the cTnI concentrations in the range of 0.44-2.2 nM exhibits a two-step increment with time. A rapid increment in early time regime up to 50 s is related with the kinetics of antibody-antigen binding reactions, and then a gradual increment is governed by the kinetics of analyte transportation. The sensor voltage variation in early time regime was equal to 70% of the total variation at 400 s.

KW - Antibody

KW - Antigen

KW - Cardiac Troponin I

KW - Hybrid AMR/PHR ring sensors

KW - Magnetic beads

KW - NC membrane

UR - http://www.scopus.com/inward/record.url?scp=81155123103&partnerID=8YFLogxK

U2 - 10.1016/j.snb.2011.08.058

DO - 10.1016/j.snb.2011.08.058

M3 - Article

AN - SCOPUS:81155123103

SN - 0925-4005

VL - 160

SP - 747

EP - 752

JO - Sensors and Actuators, B: Chemical

JF - Sensors and Actuators, B: Chemical

IS - 1

ER -

Analytes kinetics in lateral flow membrane analyzed by cTnI monitoring using magnetic method

Abstract

Bibliographical note

Keywords

Access to Document

Other files and links

Fingerprint

Cite this