Analysis of PDZ domain interactions using yeast two-hybrid and coimmunoprecipitation assays.

Hyun Woo Lee; Jaewon Ko; Eunjoon Kim

doi:10.1385/1-59745-048-0:233

Analysis of PDZ domain interactions using yeast two-hybrid and coimmunoprecipitation assays.

Hyun Woo Lee
, Jaewon Ko
, Eunjoon Kim

Department of Brain Sciences

Korea Advanced Institute of Science and Technology

Research output: Contribution to journal › Article › peer-review

3 Scopus citations

Abstract

The PDZ domain is a protein-protein interaction module that interacts with a C-terminal short peptide motif in its binding partners. A variety of methods have been used to study PDZ domain interactions. This chapter details the two methods most commonly used in the analysis of PDZ interactions: yeast two-hybrid and coimmunoprecipitation assays. In addition, we discuss the features that must be considered for an efficient analysis of PDZ interactions.

Original language	English
Pages (from-to)	233-244
Number of pages	12
Journal	Methods in molecular biology (Clifton, N.J.)
Volume	332
DOIs	https://doi.org/10.1385/1-59745-048-0:233
State	Published - 2006

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10.1385/1-59745-048-0:233

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title = "Analysis of PDZ domain interactions using yeast two-hybrid and coimmunoprecipitation assays.",

abstract = "The PDZ domain is a protein-protein interaction module that interacts with a C-terminal short peptide motif in its binding partners. A variety of methods have been used to study PDZ domain interactions. This chapter details the two methods most commonly used in the analysis of PDZ interactions: yeast two-hybrid and coimmunoprecipitation assays. In addition, we discuss the features that must be considered for an efficient analysis of PDZ interactions.",

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N2 - The PDZ domain is a protein-protein interaction module that interacts with a C-terminal short peptide motif in its binding partners. A variety of methods have been used to study PDZ domain interactions. This chapter details the two methods most commonly used in the analysis of PDZ interactions: yeast two-hybrid and coimmunoprecipitation assays. In addition, we discuss the features that must be considered for an efficient analysis of PDZ interactions.

AB - The PDZ domain is a protein-protein interaction module that interacts with a C-terminal short peptide motif in its binding partners. A variety of methods have been used to study PDZ domain interactions. This chapter details the two methods most commonly used in the analysis of PDZ interactions: yeast two-hybrid and coimmunoprecipitation assays. In addition, we discuss the features that must be considered for an efficient analysis of PDZ interactions.

UR - https://www.scopus.com/pages/publications/33747401616

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DO - 10.1385/1-59745-048-0:233

M3 - Article

C2 - 16878696

AN - SCOPUS:33747401616

SN - 1064-3745

VL - 332

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JO - Methods in molecular biology (Clifton, N.J.)

JF - Methods in molecular biology (Clifton, N.J.)

ER -

Analysis of PDZ domain interactions using yeast two-hybrid and coimmunoprecipitation assays.

Abstract

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