An overview of label-free quantitative holographic live cell imaging for drug toxicity assessment

Inkyu Moon

doi:10.1117/12.3044391

An overview of label-free quantitative holographic live cell imaging for drug toxicity assessment

Inkyu Moon

Department of Robotics and Mechatronics Engineering

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

Abstract

Quantitative phase imaging with digital holographic microscopy (QP-DHM) can be employed to quantify the movement of cardiomyocytes. In this paper, we review the application of QP-DHM in studying cardiomyocytes derived from induced pluripotent stem cells (iPSCs), in control and drug-treated conditions. This allowed us to extract a set of several parameters that can characterize the cardiomyocytes beating patterns from the recorded quantitative phase signal. We monitored the effects of E-4031 and isoprenaline on the cardiomyocyte beating patterns. Our results show that some effects specific to the mode of action of the drugs used can be identified. This stresses that QP-DHM can represent a promising label-free approach to identify new drug candidates by measuring their effects on iPSC-derived cardiomyocytes.

Original language	English
Title of host publication	Three-Dimensional Imaging, Visualization, and Display 2025
Editors	Bahram Javidi, Xin Shen, Arun Anand
Publisher	SPIE
ISBN (Electronic)	9781510687196
DOIs	https://doi.org/10.1117/12.3044391
State	Published - 2025
Event	Three-Dimensional Imaging, Visualization, and Display 2025 - Orlando, United States Duration: 14 Apr 2025 → 16 Apr 2025

Publication series

Name	Proceedings of SPIE - The International Society for Optical Engineering
Volume	13465
ISSN (Print)	0277-786X
ISSN (Electronic)	1996-756X

Conference

Conference	Three-Dimensional Imaging, Visualization, and Display 2025
Country/Territory	United States
City	Orlando
Period	14/04/25 → 16/04/25

Bibliographical note

Publisher Copyright:
© COPYRIGHT SPIE. Downloading of the abstract is permitted for personal use only.

Keywords

cardiomyocyte
Digital holographic microscopy
iPSCs
Label-free imaging
Quantitative phase imaging

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10.1117/12.3044391

Cite this

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title = "An overview of label-free quantitative holographic live cell imaging for drug toxicity assessment",

abstract = "Quantitative phase imaging with digital holographic microscopy (QP-DHM) can be employed to quantify the movement of cardiomyocytes. In this paper, we review the application of QP-DHM in studying cardiomyocytes derived from induced pluripotent stem cells (iPSCs), in control and drug-treated conditions. This allowed us to extract a set of several parameters that can characterize the cardiomyocytes beating patterns from the recorded quantitative phase signal. We monitored the effects of E-4031 and isoprenaline on the cardiomyocyte beating patterns. Our results show that some effects specific to the mode of action of the drugs used can be identified. This stresses that QP-DHM can represent a promising label-free approach to identify new drug candidates by measuring their effects on iPSC-derived cardiomyocytes.",

keywords = "cardiomyocyte, Digital holographic microscopy, iPSCs, Label-free imaging, Quantitative phase imaging",

author = "Inkyu Moon",

note = "Publisher Copyright: {\textcopyright} COPYRIGHT SPIE. Downloading of the abstract is permitted for personal use only.; Three-Dimensional Imaging, Visualization, and Display 2025 ; Conference date: 14-04-2025 Through 16-04-2025",

year = "2025",

doi = "10.1117/12.3044391",

language = "English",

series = "Proceedings of SPIE - The International Society for Optical Engineering",

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editor = "Bahram Javidi and Xin Shen and Arun Anand",

booktitle = "Three-Dimensional Imaging, Visualization, and Display 2025",

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Moon, I 2025, An overview of label-free quantitative holographic live cell imaging for drug toxicity assessment. in B Javidi, X Shen & A Anand (eds), Three-Dimensional Imaging, Visualization, and Display 2025., 134650D, Proceedings of SPIE - The International Society for Optical Engineering, vol. 13465, SPIE, Three-Dimensional Imaging, Visualization, and Display 2025, Orlando, United States, 14/04/25. https://doi.org/10.1117/12.3044391

An overview of label-free quantitative holographic live cell imaging for drug toxicity assessment. / Moon, Inkyu.
Three-Dimensional Imaging, Visualization, and Display 2025. ed. / Bahram Javidi; Xin Shen; Arun Anand. SPIE, 2025. 134650D (Proceedings of SPIE - The International Society for Optical Engineering; Vol. 13465).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

TY - GEN

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AU - Moon, Inkyu

N1 - Publisher Copyright: © COPYRIGHT SPIE. Downloading of the abstract is permitted for personal use only.

PY - 2025

Y1 - 2025

N2 - Quantitative phase imaging with digital holographic microscopy (QP-DHM) can be employed to quantify the movement of cardiomyocytes. In this paper, we review the application of QP-DHM in studying cardiomyocytes derived from induced pluripotent stem cells (iPSCs), in control and drug-treated conditions. This allowed us to extract a set of several parameters that can characterize the cardiomyocytes beating patterns from the recorded quantitative phase signal. We monitored the effects of E-4031 and isoprenaline on the cardiomyocyte beating patterns. Our results show that some effects specific to the mode of action of the drugs used can be identified. This stresses that QP-DHM can represent a promising label-free approach to identify new drug candidates by measuring their effects on iPSC-derived cardiomyocytes.

AB - Quantitative phase imaging with digital holographic microscopy (QP-DHM) can be employed to quantify the movement of cardiomyocytes. In this paper, we review the application of QP-DHM in studying cardiomyocytes derived from induced pluripotent stem cells (iPSCs), in control and drug-treated conditions. This allowed us to extract a set of several parameters that can characterize the cardiomyocytes beating patterns from the recorded quantitative phase signal. We monitored the effects of E-4031 and isoprenaline on the cardiomyocyte beating patterns. Our results show that some effects specific to the mode of action of the drugs used can be identified. This stresses that QP-DHM can represent a promising label-free approach to identify new drug candidates by measuring their effects on iPSC-derived cardiomyocytes.

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KW - Digital holographic microscopy

KW - iPSCs

KW - Label-free imaging

KW - Quantitative phase imaging

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M3 - Conference contribution

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T3 - Proceedings of SPIE - The International Society for Optical Engineering

BT - Three-Dimensional Imaging, Visualization, and Display 2025

A2 - Javidi, Bahram

A2 - Shen, Xin

A2 - Anand, Arun

PB - SPIE

T2 - Three-Dimensional Imaging, Visualization, and Display 2025

Y2 - 14 April 2025 through 16 April 2025

ER -

An overview of label-free quantitative holographic live cell imaging for drug toxicity assessment

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Keywords

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