An ISFET biosensor for the monitoring of maltose-induced conformational changes in MBP

Hye Jung Park; Sang Kyu Kim; Kyoungsook Park; Hong Kun Lyu; Chang Soo Lee; Sang J. Chung; Wan Soo Yun; Moonil Kim; Bong Hyun Chung

doi:10.1016/j.febslet.2008.11.039

An ISFET biosensor for the monitoring of maltose-induced conformational changes in MBP

Hye Jung Park, Sang Kyu Kim, Kyoungsook Park, Hong Kun Lyu, Chang Soo Lee, Sang J. Chung, Wan Soo Yun, Moonil Kim, Bong Hyun Chung

Division of Electronics & Information System

Research output: Contribution to journal › Article › peer-review

24 Scopus citations

Abstract

Here we describe an ion sensitive field effect transistor (ISFET) biosensor, which was designed to monitor directly the surface charge of structurally altered maltose binding protein (MBP) upon stimulation with maltose. This study is the first report of the application of a FET biosensor to the monitoring of conformationally changed proteins. Consequently, a significant drop in current on the basis of the charge-dependent capacitance measurement has been clearly observed in response to maltose, but not for the glucose control, thereby indicating that the substrate-specific conformational properties of the target protein could be successfully monitored using the ISFET. Collectively, our results clearly suggest that ISFET provide a high fidelity system for the detection of maltose-induced structural alterations in MBP.

Original language	English
Pages (from-to)	157-162
Number of pages	6
Journal	FEBS Letters
Volume	583
Issue number	1
DOIs	https://doi.org/10.1016/j.febslet.2008.11.039
State	Published - 5 Jan 2009

Bibliographical note

Funding Information:
This research was supported by grants from the Nano/Bio Science and Technology Program (2005-01321, MEST, Korea), and the KRIBB Initiative Research Program (KRIBB, Korea).

Keywords

Biosensor
Conformational change
ISFET
MBP
Surface charge

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10.1016/j.febslet.2008.11.039

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title = "An ISFET biosensor for the monitoring of maltose-induced conformational changes in MBP",

abstract = "Here we describe an ion sensitive field effect transistor (ISFET) biosensor, which was designed to monitor directly the surface charge of structurally altered maltose binding protein (MBP) upon stimulation with maltose. This study is the first report of the application of a FET biosensor to the monitoring of conformationally changed proteins. Consequently, a significant drop in current on the basis of the charge-dependent capacitance measurement has been clearly observed in response to maltose, but not for the glucose control, thereby indicating that the substrate-specific conformational properties of the target protein could be successfully monitored using the ISFET. Collectively, our results clearly suggest that ISFET provide a high fidelity system for the detection of maltose-induced structural alterations in MBP.",

keywords = "Biosensor, Conformational change, ISFET, MBP, Surface charge",

author = "Park, {Hye Jung} and Kim, {Sang Kyu} and Kyoungsook Park and Lyu, {Hong Kun} and Lee, {Chang Soo} and Chung, {Sang J.} and Yun, {Wan Soo} and Moonil Kim and Chung, {Bong Hyun}",

note = "Funding Information: This research was supported by grants from the Nano/Bio Science and Technology Program (2005-01321, MEST, Korea), and the KRIBB Initiative Research Program (KRIBB, Korea). ",

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doi = "10.1016/j.febslet.2008.11.039",

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T1 - An ISFET biosensor for the monitoring of maltose-induced conformational changes in MBP

AU - Park, Hye Jung

AU - Kim, Sang Kyu

AU - Park, Kyoungsook

AU - Lyu, Hong Kun

AU - Lee, Chang Soo

AU - Chung, Sang J.

AU - Yun, Wan Soo

AU - Kim, Moonil

AU - Chung, Bong Hyun

N1 - Funding Information: This research was supported by grants from the Nano/Bio Science and Technology Program (2005-01321, MEST, Korea), and the KRIBB Initiative Research Program (KRIBB, Korea).

PY - 2009/1/5

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N2 - Here we describe an ion sensitive field effect transistor (ISFET) biosensor, which was designed to monitor directly the surface charge of structurally altered maltose binding protein (MBP) upon stimulation with maltose. This study is the first report of the application of a FET biosensor to the monitoring of conformationally changed proteins. Consequently, a significant drop in current on the basis of the charge-dependent capacitance measurement has been clearly observed in response to maltose, but not for the glucose control, thereby indicating that the substrate-specific conformational properties of the target protein could be successfully monitored using the ISFET. Collectively, our results clearly suggest that ISFET provide a high fidelity system for the detection of maltose-induced structural alterations in MBP.

AB - Here we describe an ion sensitive field effect transistor (ISFET) biosensor, which was designed to monitor directly the surface charge of structurally altered maltose binding protein (MBP) upon stimulation with maltose. This study is the first report of the application of a FET biosensor to the monitoring of conformationally changed proteins. Consequently, a significant drop in current on the basis of the charge-dependent capacitance measurement has been clearly observed in response to maltose, but not for the glucose control, thereby indicating that the substrate-specific conformational properties of the target protein could be successfully monitored using the ISFET. Collectively, our results clearly suggest that ISFET provide a high fidelity system for the detection of maltose-induced structural alterations in MBP.

KW - Biosensor

KW - Conformational change

KW - ISFET

KW - MBP

KW - Surface charge

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SN - 0014-5793

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EP - 162

JO - FEBS Letters

JF - FEBS Letters

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ER -

An ISFET biosensor for the monitoring of maltose-induced conformational changes in MBP

Abstract

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Keywords

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