TY - JOUR
T1 - An Electrochemical Perspective Assay for Anticancer Activity of Calotropis Procera Against Glioblastoma Cell Line (LN-18) using Carbon Nanotubes- Graphene Nano- Conglomerate as a Podium
AU - Nalini, Seetharamaiah
AU - Nandini, Seetharamaiah
AU - Suresh, Gurukar Shivappa
AU - Melo, Jose Savio
AU - Neelagund, Shivayogeeswar E.
AU - NaveenKumar, Hunasepalya Nagaiah
AU - Sanetuntikul, Jakkid
AU - Shanmugam, Sangaraju
N1 - Publisher Copyright:
© 2016 VBRI Press.
PY - 2016
Y1 - 2016
N2 - In this article, we report the pre-screening of anti-cancer effect of Calotropis procera against glioblastoma cell lines (LN-18) by means of electrochemical methods. Soxhlet assisted extraction (SAE) has been employed to extract the polyphenol contents present in the leaf of Calotropis procera. The phytochemical analysis of the extract has been studied and the polyphenol contents were determined using Folin Ciocalteau method. To study the anticancer effects of the aqueous plant extract, a cytosensor (Gr/NT-G/LN-18) was fabricated and its possible mechanism for DNA binding was studied using graphite /poly(allylamine hydrochloride)/nanotube-graphene composite /polypyrrole /de-oxy ribonucleic acid (Gr/PAH/NT-G/PPy/DNA) modified electrode. The electrochemical characteristics of the proposed Gr/NT-G/LN-18 cytosensor towards the plant extract were evaluated using electrochemical techniques like cyclic voltammetry and differential pulse voltammetry. Scanning electron microscopy (SEM) and energy dispersive analysis of x-ray (EDAX) have been employed to study the physical characterization of the Gr/PAH/NT-G/PPy/DNA modified electrode. These results indicate that the plant extract has an ability to act as an antiglioblastoma against LN-18 cancer cells.
AB - In this article, we report the pre-screening of anti-cancer effect of Calotropis procera against glioblastoma cell lines (LN-18) by means of electrochemical methods. Soxhlet assisted extraction (SAE) has been employed to extract the polyphenol contents present in the leaf of Calotropis procera. The phytochemical analysis of the extract has been studied and the polyphenol contents were determined using Folin Ciocalteau method. To study the anticancer effects of the aqueous plant extract, a cytosensor (Gr/NT-G/LN-18) was fabricated and its possible mechanism for DNA binding was studied using graphite /poly(allylamine hydrochloride)/nanotube-graphene composite /polypyrrole /de-oxy ribonucleic acid (Gr/PAH/NT-G/PPy/DNA) modified electrode. The electrochemical characteristics of the proposed Gr/NT-G/LN-18 cytosensor towards the plant extract were evaluated using electrochemical techniques like cyclic voltammetry and differential pulse voltammetry. Scanning electron microscopy (SEM) and energy dispersive analysis of x-ray (EDAX) have been employed to study the physical characterization of the Gr/PAH/NT-G/PPy/DNA modified electrode. These results indicate that the plant extract has an ability to act as an antiglioblastoma against LN-18 cancer cells.
KW - Anticancer agents
KW - Biological activity
KW - DNA
KW - LN-18 cells
KW - Soxhlet assisted extraction
UR - http://www.scopus.com/inward/record.url?scp=84999836740&partnerID=8YFLogxK
U2 - 10.5185/amlett.2016.6395
DO - 10.5185/amlett.2016.6395
M3 - Article
AN - SCOPUS:84999836740
SN - 0976-3961
VL - 7
SP - 1003
EP - 1009
JO - Advanced Materials Letters
JF - Advanced Materials Letters
IS - 12
ER -
