Ambient Mass Spectrometry Imaging of Small Molecules from Cells and Tissues

Jae Young Kim; Heejin Lim; Dae Won Moon

doi:10.1007/978-1-0716-2030-4_3

Ambient Mass Spectrometry Imaging of Small Molecules from Cells and Tissues

Jae Young Kim, Heejin Lim, Dae Won Moon

Department of New Biology

Research output: Chapter in Book/Report/Conference proceeding › Chapter › peer-review

2 Scopus citations

Abstract

New methods to analyze cells and tissues in ambient condition without any harsh chemical fixation or physical freezing and drying are summarized in this report. The first approach, an atmospheric pressure mass spectrometry imaging method, is based on laser ablation in atmospheric pressure assisted by atmospheric plasma and nanomaterials such as nanoparticles and graphene to enhance laser ablation. The second one is based on secondary ion mass spectrometry (SIMS) imaging of live cells in solution capped with single-layer graphene to preserve intact and hydrated biological samples even under ultrahigh vacuum for SIMS bio-imaging in solution.

Original language	English
Title of host publication	Methods in Molecular Biology
Publisher	Humana Press Inc.
Pages	41-59
Number of pages	19
DOIs	https://doi.org/10.1007/978-1-0716-2030-4_3
State	Published - 2022

Publication series

Name	Methods in Molecular Biology
Volume	2437
ISSN (Print)	1064-3745
ISSN (Electronic)	1940-6029

Bibliographical note

Publisher Copyright:
© 2022, The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.

Keywords

Ambient mass spectrometric imaging
Atmospheric pressure mass spectrometry imaging
Laser ablation
Nanomaterials
Secondary ion mass spectrometry
Single-layer graphene

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10.1007/978-1-0716-2030-4_3

Cite this

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abstract = "New methods to analyze cells and tissues in ambient condition without any harsh chemical fixation or physical freezing and drying are summarized in this report. The first approach, an atmospheric pressure mass spectrometry imaging method, is based on laser ablation in atmospheric pressure assisted by atmospheric plasma and nanomaterials such as nanoparticles and graphene to enhance laser ablation. The second one is based on secondary ion mass spectrometry (SIMS) imaging of live cells in solution capped with single-layer graphene to preserve intact and hydrated biological samples even under ultrahigh vacuum for SIMS bio-imaging in solution.",

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AU - Kim, Jae Young

AU - Lim, Heejin

AU - Moon, Dae Won

PY - 2022

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AB - New methods to analyze cells and tissues in ambient condition without any harsh chemical fixation or physical freezing and drying are summarized in this report. The first approach, an atmospheric pressure mass spectrometry imaging method, is based on laser ablation in atmospheric pressure assisted by atmospheric plasma and nanomaterials such as nanoparticles and graphene to enhance laser ablation. The second one is based on secondary ion mass spectrometry (SIMS) imaging of live cells in solution capped with single-layer graphene to preserve intact and hydrated biological samples even under ultrahigh vacuum for SIMS bio-imaging in solution.

KW - Ambient mass spectrometric imaging

KW - Atmospheric pressure mass spectrometry imaging

KW - Laser ablation

KW - Nanomaterials

KW - Secondary ion mass spectrometry

KW - Single-layer graphene

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DO - 10.1007/978-1-0716-2030-4_3

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T3 - Methods in Molecular Biology

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EP - 59

BT - Methods in Molecular Biology

PB - Humana Press Inc.

ER -

Ambient Mass Spectrometry Imaging of Small Molecules from Cells and Tissues

Abstract

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