Abstract
Mood disorders and antidepressant therapy involve alterations of monoaminergic and glutamatergic transmission. The protein S100A10 (p11) was identified as a regulator of serotonin receptors, and it has been implicated in the etiology of depression and in mediating the antidepressant actions of selective serotonin reuptake inhibitors. Here we report that p11 can also regulate depression-like behaviors via regulation of a glutamatergic receptor in mice. p11 directly binds to the cytoplasmic tail of metabotropic glutamate receptor 5 (mGluR5). p11 and mGluR5 mutually facilitate their accumulation at the plasma membrane, and p11 increases cell surface availability of the receptor. Whereas p11 overexpression potentiates mGluR5 agonist-induced calcium responses, overexpression of mGluR5 mutant, which does not interact with p11, diminishes the calcium responses in cultured cells. Knockout of mGluR5 or p11 specifically in glutamatergic neurons in mice causes depression-like behaviors. Conversely, knockout of mGluR5 or p11 in GABAergic neurons causes antidepressant-like behaviors. Inhibition of mGluR5 with an antagonist, 2-methyl-6-(phenylethynyl)pyridine (MPEP), induces antidepressant-like behaviors in a p11-dependent manner. Notably, the antidepressant-like action of MPEP is mediated by parvalbumin-positive GABAergic interneurons, resulting in a decrease of inhibitory neuronal firing with a resultant increase of excitatory neuronal firing. These results identify a molecular and cellular basis by which mGluR5 antagonism achieves its antidepressant-like activity.
| Original language | English |
|---|---|
| Pages (from-to) | 1546-1556 |
| Number of pages | 11 |
| Journal | Molecular Psychiatry |
| Volume | 20 |
| Issue number | 12 |
| DOIs | |
| State | Published - 1 Dec 2015 |
Bibliographical note
Funding Information:We thank Dr H Wang for discussion and sharing materials. We thank Dr A Contractor for providing us floxed mGluR5 mice. We thank C Hsaio for assistance with animal maintenance. We acknowledge R Norinsky and the Rockefeller University Transgenics Services Laboratory for their excellent in vitro fertilization services, and H Zebroski III and the Rockefeller University Proteomics Resource Center for peptide synthesis. We thank Drs K Thomas and A North at the Rockefeller University Bio-imaging Resource Center for their help with fluorescent microscopy. We acknowledge E Griggs for graphics. This work was supported by DOD/USAMRMC Grants W81XWH-09-1-0392 (to YK), W81XWH-10-1-0691 (to MF) and W81XWH-09-1-0402 (to PG); NIH Grants MH074866 (to PG) and DA010044 (to PG); the Fisher Center for Alzheimer’s Research Foundation (to PG); The JPB Foundation (to PG); Swedish Research Council and the Erling-Persson Family Foundation (to AA); and the National Leading Research Laboratory Program, 2011-0028772 (to DK).
